Anti-GABARAP Antibody | Alomone Labs AIP-016 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GABARAP Antibody

catalog :

AIP-016

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain (lanes 1 and 4), rat brain (lanes 2 and 5) and human neuroblastoma (SH-SY5Y) cell line (lanes 3 and 6) lysates: - 1-3. Anti-GABARAP Antibody (#AIP-016), (1:800).4-6. Anti-GABARAP Antibody, preincubated with GABARAP Blocking Peptide (#BLP-IP016).

image 2 :

Expression of GABARAP in rat cortex. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GABARAP Antibody (#AIP-016), (1:1000), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. GABARAP immunoreactivity (red) appears in cortical neurons (arrows). B. Pre-incubation of the antibody with GABARAP Blocking Peptide (#BLP-IP016), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AIP-016

SKU :

AIP-016-CF_0.2 ml

Product Name :

Anti-GABARAP Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P60517

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-IP016

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KFVYKEEHPFEKRR, corresponding to amino acid residues 2-15 of rat GABARAP

Product Page - Scientific background :

GABA(A) receptors are ligand-gated chloride channels that mediate inhibitory neurotransmission. GABA associated protein (GABARAP) family interacts specifically with the γ1, γ2S and γ2L subunits of the GABA(A) receptor. GABARAP is expressed in a variety of tissues including heart, brain, placenta, liver and others. The genetic sequence encoding GABARAP is similar to microtubule-associated proteins 1A and 1B1.GABARAP has an asymmetric structure with a basic N-terminal region and an acidic C terminus. GABARAP has a compact fold consisting of four or five-stranded β-sheets with two α-helices on each side. The outer strands of the β-sheets are aligned anti-parallel to the inner strands and helices α3 and α4 are located on one side of the sheet. The presence of α1 and α2 is a unique feature of GABARAP and forms a hydrophilic patch suitable for protein-protein interface. GABARAP exists in a monomeric conformation at low protein concentration and a head-to-tail oligomeric conformation in high salt conditions.GABARAP is involved in intracellular trafficking and is an integral part of the intra-Golgi transport machinery. Thus it is detected in cellular compartments such as the Golgi apparatus and endoplasmic reticulum. GABARAP affects GABA(A) receptors' cell surface expression by increasing their intracellular trafficking. Disruption of cellular microtubules prevents the clustering of both GABARAP and GABA(A) receptors. GABARAP also interacts with other receptors such as the glycine receptor at the postsynaptic site2.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

GABA receptor associated protein, GABARAPL

Short Description :

A Rabbit Polyclonal Antibody to GABA Receptor Associated Protein

Long Description :

Anti-GABARAP Antibody (#AIP-016) is a highly specific antibody directed against an epitope of the rat GABA receptor associated protein. The antibody can be used in western blot analysis. It has been designed to recognize GABARAP from human, mouse and rat samples. Due to high homology with GABA receptor associated protein-like proteins the antibody will recognize GABA receptor associated protein-like 1 and 3 and may recognize GABA receptor associated protein-like 2.

Negative Control :

BLP-IP016

Positive Control :

Synonyms :

GABA receptor associated protein, GABARAPL

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GABARAP

AB Specifictiy :

Due to high homology with GABA receptor associated protein-like proteins the antibody will recognize:GABA receptor associated protein-like 1 (11/14 amino acid residues identical), (Accession Q0VGK0).GABA receptor associated protein-like 3 (11/14 amino acid residues identical), (Accession Q9BY60).May recognize GABA receptor associated protein-like 2 (8/14 amino acid residues identical), (Accession P60522).

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:1000

Application Dilutions: Western blot wb :

1:800

more info or order :

Alomone Labs product webpage