Anti-PACSIN3 Antibody | Alomone Labs AIP-015 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-PACSIN3 Antibody

catalog :

AIP-015

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Crowley L, Cambuli F, Aparicio L, Shibata M, Robinson B, Xuan S, et al. A single-cell atlas of the mouse and human prostate reveals heterogeneity and conservation of epithelial progenitors. elife. 2020;9: pubmed publisher

image

image 1 :

Western blot analysis of rat brain membrane (lanes 1 and 5), rat skeletal muscle lysate (lanes 2 and 6), rat heart lysate (lanes 3 and 7) and mouse heart membrane (lanes 4 and 8): - 1-4. Anti-PACSIN3 Antibody (#AIP-015), (1:400).5-8. Anti-PACSIN3 Antibody, preincubated with PACSIN3 Blocking Peptide (#BLP-IP015).

product information

CAT :

AIP-015

SKU :

AIP-015-CF_0.2 ml

Product Name :

Anti-PACSIN3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q99JB8

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-IP015

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RKDEKTAQTRESHAK, corresponding to amino acid residues 161-175 of mouse Pacsin3

Product Page - Scientific background :

Pacsin 3 (also known as Syndapin 3) is a member of the highly conserved Pacsin protein sub-family which constitutes a branch of the larger F-BAR domain protein family.Pacsin 3, as well as other members of its family, is a cytoplasmic protein involved in receptor-mediated endocytosis, synaptic vesicle trafficking and biogenesis of different cellular organelles and is mostly, but not only, expressed in lung and muscle tissues. Pacsin 3 contains an N-terminal F-BAR domain and a C-terminal mono-Src homology 3 domain1.The C-terminal SH3 domain of Pacsin 3 interacts with the N-terminal proline-rich domain of TRPV4 at residues 132-144 and enhances the cell surface expression of TRPV4, probably through the inhibition of the endocytotic process2. The SH3 domain is also responsible for interactions with the protein dynamin and with N-WASP, a potent activator of the Arp2/3 complex F-actin-nucleation machine3. Pacsin's interaction with dynamin contributes to fusion pore expansion in chromaffin cells in the adrenal medulla. Pacsin-dynamin interaction occurs through an SH3-PRD binding interaction that depends on the central proline residue in the ligand-binding site of Pacsin's SH3 domain4.Pacsin 3 is also involved in the trafficking of AMPA receptors in neurons through its interaction with PICK1 (Protein interacting with C-kinase 1). PICK1 and Pacsin 3 form a complex with the C-termini of the AMPA subunits GluA2 and GluA3. This interaction between Pacsin 3 and PICK1 is regulated by Pacsin phosphorylation and is required for NMDA induced AMPA receptor endocytosis and cerebellar long term synaptic depression5.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Protein kinase C and casein kinase II substrate protein 3, SH3 domain-containing protein 6511, Syndapin III, SDPIII

Short Description :

A Rabbit Polyclonal Antibody to PACSIN 3

Long Description :

Anti-PACSIN3 Antibody (#AIP-015) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize Pacsin 3 from rat, mouse, and human samples.

Negative Control :

BLP-IP015

Positive Control :

Synonyms :

Protein kinase C and casein kinase II substrate protein 3, SH3 domain-containing protein 6511, Syndapin III, SDPIII

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PACSIN3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage