Anti-RIM1 Antibody | Alomone Labs AIP-014 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-RIM1 Antibody

catalog :

AIP-014

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-RIM1 Antibody (#AIP-014), (1:200).3,4. Anti-RIM1 Antibody, preincubated with RIM1 Blocking Peptide (#BLP-IP014).

product information

CAT :

AIP-014

SKU :

AIP-014-CF_0.2 ml

Product Name :

Anti-RIM1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JIR4

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-IP014

Homology :

Mouse - 15/16 amino acid residues identical; human - 14/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RAKRGRSAESLHMTSE, corresponding to amino acid residues 1039-1054 of rat RIM1

Product Page - Scientific background :

RIM1 is a member of the RIM superfamily of proteins (Rim1-4). These genes express seven known RIM isoforms: RIM1α, 1β; RIM2α, 2β, 2γ; and RIM3γ and RIM4γ which serve as scaffolding proteins in presynaptic nerve terminals by interacting with several other protein components localized at the active zone, including Munc13, ELKS (or CAST), liprins and voltage-gated Ca2+ channels (VGCCs). RIM1 directly binds to the C-terminal regions of the α1 subunit of N- and P/Q-type calcium channels, and can also interact with the β auxiliary subunit of P/Q-type calcium channels1-3.RIM proteins were found to be required for normal Ca2+ triggering of exocytosis. This interaction with Ca2+ channel significantly slows channel inactivation resulting in increased Ca2+ influx during action potentials.Deficiency of RIM1 decreases presynaptic Ca2+ channel density. RIM proteins are involved in neurotransmitter release and contribute to long- and short-term synaptic plasticity.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Regulating synaptic membrane exocytosis 1, Rab-3-interacting molecule 1, RIMS1

Short Description :

A Rabbit Polyclonal Antibody to RIM1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat RIM1. Anti-RIM1 Antibody (#AIP-014) can be used in western blot analysis. It has been designed to recognize RIM1 from mouse, rat and human samples.

Negative Control :

BLP-IP014

Positive Control :

Synonyms :

Regulating synaptic membrane exocytosis 1, Rab-3-interacting molecule 1, RIMS1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

RIMS1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage