Anti-Presenilin-2 Antibody | Alomone Labs AIP-012 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Presenilin-2 Antibody

catalog :

AIP-012

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat heart membrane: - 1. Anti-Presenilin-2 Antibody (#AIP-012), (1:400). 2. Anti-Presenilin-2 Antibody, preincubated with Presenilin-2 Blocking Peptide (#BLP-IP012).

image 2 :

Expression of Presenilin-2 in mouse midbrain - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Presenilin-2 Antibody (#AIP-012), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Presenilin-2 immunoreactivity (green) appears in neurons along the midline (horizontal arrows) and away from midline (vertical arrows). B. Pre-incubation of the antibody with Presenilin-2 Blocking Peptide (BLP-IP012), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AIP-012

SKU :

AIP-012-CF_0.2 ml

Product Name :

Anti-Presenilin-2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O88777

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-IP012

Homology :

Mouse, human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DSRPGPEDGENTAQWR, corresponding to amino acid residues 33-48 of rat Presenilin-2

Product Page - Scientific background :

Presenilin 2 (PS2) is the catalytic subunit of a tetrameric complex containing presenilin-1 or 2, anterior pharynx defective 1 (APH1), nicastrin, and PEN-21.The presenilin complex is the founding member of a unique class of GXGD aspartyl proteases that catalyze the cleavage of the transmembrane domains of Type I membrane proteins including amyloid precursor protein (APP) and Notch1. Other members of this protease family include signal peptide peptidases (SPP) and a variety of archaeal homologs2.PS1 and PS2 proteins have nine helical TM domains arranged with the hydrophilic, flexible N terminus in the cytosol and the C terminus protruding into the lumen or extracellular space3.PS2 shares similarity with PS 1 and the crucial difference between them appears to be a "[DIE]xxxL/I/M" motif present only in PS2 which interacts in a phosphorylation dependent manner with AP-1 complexes and targets PS2 to the late endosome/lysosome complex. The absence of this sequence motif in PS1 allows for the wider subcellular location of PS1 in cell membranes4.Presenilin mutations have been shown to be correlated with occurrence of familial Alzheimer's disease5.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Psen2, Ps2, Psnl2, AD3LP, AD5, E5-1 STM-2

Short Description :

A Rabbit Polyclonal Antibody to Presenilin-2

Long Description :

Anti-Presenilin-2 Antibody (#AIP-012) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize PSEN2 from rat, mouse, and human samples.

Negative Control :

BLP-IP012

Positive Control :

Synonyms :

Psen2, Ps2, Psnl2, AD3LP, AD5, E5-1 STM-2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PSEN2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage