Anti-Presenilin-1 Antibody | Alomone Labs AIP-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Presenilin-1 Antibody

catalog :

AIP-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat pancreas membrane (lanes 1 and 4), rat hippocampus (lanes 2 and 5) and mouse pancreatic cancer cell line (MS1) (lanes 3 and 6): - 1-3. Anti-Presenilin-1 Antibody (#AIP-011), (1:500).4-6. Anti-Presenilin-1 Antibody, preincubated with Presenilin-1 Blocking Peptide (#BLP-IP011).

image 2 :

Expression of Presenilin-1 in mouse brain - Immunohistochemical staining of mouse hippocampus and mouse cerebellum using Anti-Presenilin-1 Antibody (#AIP-011), (1:600). A. In hippocampus, PS-1 staining (green) appears in apical dendrites of pyramidal neurons (arrows). B. In cerebellum, an intense staining for PS-1 (green) appears in dendritic trees of Purkinje cells (arrows). A moderate staining is detected in the granule layer and in the soma of Purkinje and molecular layer interneurons. In both panels DAPI is used as the counterstain.

product information

CAT :

AIP-011

SKU :

AIP-011-CF_0.2 ml

Product Name :

Anti-Presenilin-1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P97887

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-IP011

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd cytoplasmic loop (at the Psen1 CTF subunit)

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RDSHLGPHRSTPESR, corresponding to amino acid residues 345-359 of rat Psen1

Product Page - Scientific background :

Presenilin-1 (PSEN1) is a transmembrane protein encoded by the PS1 gene. The protein is comprised of 9 transmembrane domains. The N- and C-termini of the protein are cytosolic and lumenal respectively. PSEN1, together with three other proteins - nicastrin, presenilin enhancer 2 and anterior pharynx-defective 1 form a protein complex named γ-Secretase. PSEN1 serves as the catalytic subunit of the γ-secretase complex. This complex, along with α- and β-secretases cleaves the amyloid precursor protein (APP). APP is the precursor for β-Amyloid fibrils which are the pathological hallmark of Alzheimer's disease (AD) and mutations in the PSEN1 gene have been implicated in AD pathophysiology. Currently, it remains unclear whether PSEN1 mutations cause disease by a loss of function or a gain of toxic function mechanism1.PS1 mutations causing an overexpression of mutant human PSEN1 also increase the expression of ryanodine receptor 3 in PC12 cells. In addition, PC12 and cortical neuron cells expressing mutant PSEN1 exhibit increased calcium responses to caffeine compared with cells expressing wildtype PSEN1. This enhanced release of calcium is associated with increased cell vulnerability to β-Amyloid and caffeine induced cellular death. It has been hypothesized that PSEN1 and RyR interact directly2.PS1 mutations also enhance inositol triphosphate (IP3)-mediated Ca2+ release in non-excitable and excitable cells. IP3-evoked Ca2+ responses are more than threefold greater in PS1M146V knock-in mice relative to non-transgenic controls. These mutations specifically disrupt intracellular Ca2+ release rather than reduce cytosolic Ca2+ buffering or clearance3.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

PSEN1, PS-1, Psnl1

Short Description :

A Rabbit Polyclonal Antibody to Presenilin-1

Long Description :

Anti-Presenilin-1 Antibody (#AIP-011) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize PSEN1 from rat, mouse, and human samples.

Negative Control :

BLP-IP011

Positive Control :

Synonyms :

PSEN1, PS-1, Psnl1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PSEN1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:600

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage