antibody

Anti-Presenilin-1-ATTO Fluor-488 Antibody

AIP-011-AG

polyclonal

domestic rabbit

ATTO 488

NA

human, mouse, rat

immunohistochemistry, immunocytochemistry

AIP-011-AG_50 mcl

Anti-Presenilin-1-ATTO Fluor-488 Antibody

Antibodies

Antibodies

Polyclonal

P97887

IF IHC

Human Rat Mouse

Rabbit

BLP-IP011

Mouse, human - identical

PBS pH7.4, 1% BSA with 0.05% sodium azide

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

50 µl double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1% BSA, 0.05% NaN3

3rd cytoplasmic loop (at the Psen1 CTF subunit)

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

(C)RDSHLGPHRSTPESR, corresponding to amino acid residues 345-359 of rat Psen1

Presenilin-1 (PSEN1) is a transmembrane protein encoded by the PS1 gene. The protein is comprised of 9 transmembrane domains. The N- and C-termini of the protein are cytosolic and lumenal respectively. PSEN1, together with three other proteins- nicastrin, presenilin enhancer 2 and anterior pharynx-defective 1 form a protein complex named γ-Secretase. PSEN1 serves as the catalytic subunit of the γ-secretase complex. This complex, along with α- and β-secretases cleaves the amyloid precursor protein (APP). APP is the precursor for β-Amyloid fibrils which are the pathological hallmark of Alzheimer's disease (AD) and mutations in the PSEN1 gene have been implicated in AD pathophysiology. Currently, it remains unclear whether PSEN1 mutations cause disease by a loss of function or a gain of toxic function mechanism1.PS1 mutations causing an overexpression of mutant human PSEN1 also increase the expression of ryanodine receptor 3 in PC12 cells. In addition, PC12 and cortical neuron cells expressing mutant PSEN1 exhibit increased calcium responses to caffeine compared with cells expressing wildtype PSEN1. This enhanced release of calcium is associated with increased cell vulnerability to β-Amyloid and caffeine induced cellular death. It has been hypothesized that PSEN1 and RyR interact directly2.PS1 mutations also enhance inositol triphosphate (IP3)-mediated Ca2+ release in non-excitable and excitable cells. IP3-evoked Ca2+ responses are more than threefold greater in PS1M146V knock-in mice relative to non-transgenic controls. These mutations specifically disrupt intracellular Ca2+ release rather than reduce cytosolic Ca2+ buffering or clearance3.

IF IHC

Alomone Labs

PSEN1, PS-1, Psnl1

A Rabbit Polyclonal Antibody to Presenilin-1 Conjugated to the Fluorescent Dye ATTO-488

Anti-Presenilin-1 Antibody (#AIP-011) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize PSEN1 from rat, mouse, and human samples. Anti-Presenilin-1-ATTO Fluor-488 Antibody (#AIP-011-AG) is directly labeled with ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Presenilin-1-ATTO Fluor-488 Antibody has been tested in immunohistochemistry applications and is specially suited to experiments requiring simultaneous labeling of different markers.

RIC-001-AG

NA

PSEN1, PS-1, Psnl1

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

PSEN1

Lyophilized powder

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis (unlabeled antibody, #AIP-011), and immunohistochemical staining (labeled antibody).

1 µg peptide per 1 µg antibody

1:60

Contact Alomone for information