Anti-Sigma-1 Receptor Antibody | Alomone Labs AIP-006 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Sigma-1 Receptor Antibody

catalog :

AIP-006

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) liver membranes: - 1,2. Anti-Sigma-1 Receptor Antibody (AIP-006), (1:200).3,4. Anti-Sigma-1 Receptor Antibody, preincubated with Sigma-1 Receptor Blocking Peptide (#BLP-IP006).

image 2 :

Expression of Sigma-1 Receptor in rat spinal cord. - Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-Sigma-1 Receptor Antibody (#AIP-006), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Sigma-1 Receptor immunoreactivity (green) appeared in the superficial layer of the dorsal horn (arrows). B. Pre-incubation of the antibody with Sigma-1 Receptor Blocking Peptide (BLP-IP006), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AIP-006

SKU :

AIP-006-CF_0.2 ml

Product Name :

Anti-Sigma-1 Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q99720

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-IP006

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)SRGHSGRYWAEISD, corresponding to amino acid residues 113-126 of human SIGMAR1

Product Page - Scientific background :

Sigma receptors were first identified as a subtype of opioid receptors. The different members of the family are differentially distributed in various parts of the CNS and are responsible for a vast range of functions and behaviors1. Today, sigma receptors are mostly considered to be a separate receptor family with two main subtypes characterized.The sigma-1 receptor is encoded by a gene baring the same name and is a protein of 223 amino acids with two transmembrane domains and a typical endoplasmic reticulum localized signal near its short N terminus. Sigma receptors are mostly detected in the central nervous system with a high density in the spinal cord, pons, medulla oblongata, red nucleus, cerebellum and hippocampus.Sigma-1 is mainly localized on the mitochondrial associated endoplasmic reticulum membrane (MAM), forming a BiP chaperone structure with high sensitivity to the calcium ion. When activated by agonists such as cocaine or analgesics, sigma-1 receptors separate from BiP and translocate from MAM to other parts of the cell. Through regulation of inositol triphosphate (IP3) receptors, NMDA receptors, dopamine receptors and voltage-gated Ca2+ channels, sigma-1 receptors can influence TCA cycle, oxidative stress, mitochondrial function, neuron plasticity and the release of various neurotransmitters.The exact etiology and pathophysiology of Alzheimer's disease is not fully understood. A few hypotheses suggest the involvement of Sigma-1 in the progression of Alzheimer's disease. The suggested mechanisms include protection from Aβ toxicity, prevention of Tau protein hyperphosphorylation and the upregulation of acetylcholine release2.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

SIGMAR1, OPRS1, Sigma non-opioid intracellular receptor 1, SIG-1R, Aging-associated gene 8 protein, SR31747-binding protein, SRBP

Short Description :

A Rabbit Polyclonal Antibody to Sigma-1 Receptor

Long Description :

Anti-Sigma-1 Receptor Antibody (#AIP-006) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize SIGMAR1 from human, rat, and mouse samples.

Negative Control :

BLP-IP006

Positive Control :

Synonyms :

SIGMAR1, OPRS1, Sigma non-opioid intracellular receptor 1, SIG-1R, Aging-associated gene 8 protein, SR31747-binding protein, SRBP

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SIGMAR1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage