Anti-Gephyrin Antibody | Alomone Labs AIP-005 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Gephyrin Antibody

catalog :

AIP-005

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Wang H, Cheng K, Chen P, Tseng K, Kwan A, Chang L. Glycine receptors expression in rat spinal cord and dorsal root ganglion in prostaglandin E2 intrathecal injection models. BMC Neurosci. 2018;19:72 pubmed publisher
Parker S, Moutal A, Cai S, Chandrasekaran S, Roman M, Koshy A, et al. High Fidelity Cryopreservation and Recovery of Primary Rodent Cortical Neurons. Eneuro. 2018;5: pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-Gephyrin Antibody (#AIP-005), (1:200).3,4. Anti-Gephyrin Antibody, preincubated with Gephyrin Blocking Peptide (#BLP-IP005).

image 2 :

Expression of Gephyrin in rat hippocampus - Immunohistochemical staining of rat hippocampal dentate gyrus sections using Anti-Gephyrin Antibody (#AIP-005). A. Gephyrin staining (green) appears in hilus of the dentate gyrus (arrows). B. Nuclear staining using DAPI as the counterstain (blue). C. Merge image of panel A and B.

image 3 :

Alomone Labs Cono AIVGRPRF-amide positively modulates ASIC3 channels expressed inXenopusoocytes. - A. Representative time course of Cono AIVGRPRF-amide (#STC-174) potentiation of ASIC3 current. Membrane potential was held at -80 mV current was elicited every 100 sec by a transient application of pH 5.5 and reversibly potentiated by a 60 sec application of 50 M Cono AIVGRPRF-amide as indicated. B. Superimposed traces of ASIC3 currents before (black) and after (green) application of 50 M Cono AIVGRPRF-amide which extends the current following stimulation (arrow). Taken from the experiment in A.

product information

CAT :

AIP-005

SKU :

AIP-005-CF_0.2 ml

Product Name :

Anti-Gephyrin Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q03555

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-IP005

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RDAIVKVKEVHDE, corresponding to amino acid residues 183-195 of rat Gephyrin

Product Page - Scientific background :

Gephyrin is a highly conserved, intracellular scaffolding protein responsible for the post-synaptic anchoring and assembly of the inhibitory glycine and γ-aminobutyric acid type A receptors (GlyR and GABAAR); it essentially anchors the receptors, by auto-oligomerization, to the cytoskeleton1,2.GABAergic transmission is important for the mediation of neuronal excitability and has a crucial role in neurodevelopment, throughout the central and peripheral nervous systems (especially via GABAAR). Glycinergic neurons on the other hand control motor functions and sensory signaling as well as take part in pain transmission in the spine's dorsal horn. Importantly, gephyrin has a 1:1 stoichiometry with GlyR3,4.Up-to-date atomic force microscopy data of gephyrin has shown that it forms trimers with multiple conformations. It is a modular construct with three domains: an N-terminal G-, a central C- and a C-terminal E-domain. Recent work suggests also that gephyrin regulates neuronal differentiation by anchoring small cytoskeletal-related signaling molecules at unique subcellular locations2,5,6.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

GPHN, Putative glycine receptor-tubulin linker protein, MPT adenylyltransferase, MPT Mo-transferase

Short Description :

A Rabbit Polyclonal Antibody to Gephyrin

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Gephyrin. Anti-Gephyrin Antibody (#AIP-005) can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPHN from rat, mouse, and human samples.

Negative Control :

BLP-IP005

Positive Control :

Synonyms :

GPHN, Putative glycine receptor-tubulin linker protein, MPT adenylyltransferase, MPT Mo-transferase

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPHN

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage