Anti-GPR109A/HCAR2 (extracellular) Antibody | Alomone Labs AHR-012 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR109A/HCAR2 (extracellular) Antibody

catalog :

AHR-012

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat spleen lysates (lanes 1 and 3) and rat colon membranes (lanes 2 and 4): - 1-2. Anti-GPR109A/HCAR2 (extracellular) Antibody (#AHR-012), (1:200). 3-4. Anti-GPR109A/HCAR2 (extracellular) Antibody, preincubated with GPR109A/HCAR2 (extracellular) Blocking Peptide (BLP-HR012).

image 2 :

Expression of GPR109A in rat cingulate cortex. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR109A/HCAR2 (extracellular) Antibody (#AHR-012), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. GPR109A immunoreactivity (green) appears in outlines of cortical neurons (arrows). B. Pre-incubation of the antibody with GPR109A/HCAR2 (extracellular) Blocking Peptide (BLP-HR012), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AHR-012

SKU :

AHR-012-CF_0.2 ml

Product Name :

Anti-GPR109A/HCAR2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9EP66

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-HR012

Homology :

Rat - 11 out of 13 amino acid residues identical; Human - 9 out of 13 amino acid residues identical Note: it is not expected to recognize the highly homologous human protein HCAR3 (Accession P49019) HCAR3 has no mouse or rat orthologs

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, 1st loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)DNYVHNWDWRFGG, corresponding to amino acid residues 82 - 94 of mouse HCAR2

Product Page - Scientific background :

Hydroxy-carboxylic acid receptors (HCA1-3) are a family of G-protein-coupled receptors that regulate lipolysis. HCAR2 (GPR109A) was identified as the receptor for nicotinic acid (niacin; vitamin B3) and beta-hyroxybutyrate (β-OHB), a ketone body synthesized by the liver under negative energy balance1.HCAR2 is mainly expressed on adipocytes and immune cells and in lower levels in retinal pigmented and colonic epithelial cells, microglia and normal mammary tissue. High levels of HCAR2 can be found in immune cells such as macrophages in response to the presence of interferon-γ, TNF-α and hypoxia2.HCAR2 activity in adipocytes promotes inhibition of adenylate cyclase and decrease cAMP levels. HCAR2 activity leads to a rapid decrease in lipolysis and reduction in the release of fatty acids from the adipocytes3.Activation of HCAR2 has also been associated with anti-inflammatory effects in different disease states such as sepsis, diabetes and obesity. Interaction of HCAR2 with niacin and β-OHB under this conditions lead to variety of signaling events involving inflammatory downstream targets4.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Hydroxycarboxylic Acid Receptor 2, Niacin Receptor 1, G-Protein Coupled Receptor HM74A, HM74A, Nicotinic acid receptor, PUMAG

Short Description :

A Rabbit Polyclonal antibody to GPR109A/HCAR2 (extracellular)

Long Description :

Anti-GPR109A/HCAR2 (extracellular) Antibody (#AHR-012) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize GPR109A from rat, mouse and human samples.

Negative Control :

BLP-HR012

Positive Control :

Synonyms :

Hydroxycarboxylic Acid Receptor 2, Niacin Receptor 1, G-Protein Coupled Receptor HM74A, HM74A, Nicotinic acid receptor, PUMAG

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HCAR2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage