Anti-GPR81/HCAR1 Antibody | Alomone Labs AHR-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR81/HCAR1 Antibody

catalog :

AHR-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat kidney (lanes 1 and 3) and rat liver (lanes 2 and 4) lysates: - 1, 2. Anti-GPR81/HCAR1 Antibody (#AHR-011), (1:200). 3, 4. Anti-GPR81/HCAR1 Antibody, preincubated with GPR81/HCAR1 Blocking Peptide (#BLP-HR011) (#BLP-HR011).

image 2 :

Expression of HCAR1 in mouse hippocampus. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR81/HCAR1 Antibody (#AHR-011), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. HCAR1 immunoreactivity (green) appears in hippocampal blood vessels (horizontal arrows) and astrocytes (vertical arrows). B. Pre-incubation of the antibody with GPR81/HCAR1 Blocking Peptide (BLP-HR011), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AHR-011

SKU :

AHR-011-CF_0.2 ml

Product Name :

Anti-GPR81/HCAR1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8C131

Applications :

IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-HR011

Homology :

Rat - 9/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

CSKSSIDGANRSQR, corresponding to amino acid residues 319-332 of mouse GPR81 (HCAR1)

Product Page - Scientific background :

GPR81 is an orphan G-protein-coupled receptor that belongs to a family of hydroxy-carboxylic acid (HCA) receptors. Its endogenous ligand is L-lactate, a metabolite produced when glycolysis exceeds the capacity to oxidize pyruvate1,2.In addition to being a by-product of metabolism, lactate plays a role in insulin signaling, particularly in insulin mediated anti-lipolytic effects. Likewise, GPR81 is mostly expressed in adipose tissues and its activation results in inhibition of lipolysis and reduced hydrolysis of triglycerides to glycerol and fatty acids, suggesting that GPR81 may be an attractive drug discovery target for metabolic disorders4.Beyond the control of adipocyte lipolysis, GPR81 is also involved in neuronal signaling, regulation of blood flow to the retina. The receptor is overexpressed in several cancer types where it appears to play a role in the adaptation to the ischemic tumor microenvironment5.GPR81, like all other G protein coupled receptors, consists of seven transmembrane domains, in addition it possesses a highly conserved Cys-Glu-Ser-Phe motif in the second extracellular loop, essential for its function and six Cys residues at the extracellular domains that potentially form three disulfide bonds6.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Hydroxycarboxylic acid receptor 1, HCA1 Receptor, G-protein coupled receptor 81, GPR81, G Protein-Coupled Receptor 104, LACR1

Short Description :

A Rabbit Polyclonal Antibody to GPR81

Long Description :

Anti-GPR81/HCAR1 Antibody (#AHR-011) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize HCAR1 from rat and mouse samples. The antibody will not recognize human GPR81.

Negative Control :

BLP-HR011

Positive Control :

Synonyms :

Hydroxycarboxylic acid receptor 1, HCA1 Receptor, G-protein coupled receptor 81, GPR81, G Protein-Coupled Receptor 104, LACR1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HCAR1

AB Specifictiy :

The antibody will not recognize human GPR81 (HCAR1).

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage