Anti-Histamine H1 Receptor/HRH1 (extracellular) Antibody | Alomone Labs AHR-006 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Histamine H1 Receptor/HRH1 (extracellular) Antibody

catalog :

AHR-006

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain membranes (lanes 1 and 5), rat brain membranes (lanes 2 and 6), rat lung membranes (lanes 3 and 7) and mouse lung membranes (lanes 4 and 8): - 1-4. Anti- Histamine H1 Receptor/HRH1 (extracellular) Antibody (#AHR-006), (1:200). 5-8. Anti-Histamine H1 Receptor/HRH1 (extracellular) Antibody, preincubated with Histamine H1 Receptor/HRH1 (extracellular) Blocking Peptide (#BLP-HR006).

image 2 :

Expression of Histamine H1 Receptor in mouse substantia nigra - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Histamine H1 Receptor/HRH1 Antibody (extracellular) (#AHR-006), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Histamine H1 Receptor immunoreactivity (green) appears in neuronal profiles (vertical arrows). B. Pre-incubation of the antibody with Histamine H1 Receptor (HRH1) (extracellular) Blocking Peptide (#BLP-HR006), suppresses staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AHR-006

SKU :

AHR-006-CF_0.2 ml

Product Name :

Anti-Histamine H1 Receptor/HRH1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P70174

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-HR006

Homology :

Mouse and rat - 15/16 amino acid residues identical; human - 14/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)REDK(S)ETDFYNVTWFK, corresponding to amino acid residues 176 - 191 of mouse H1 Histamine Receptor

Product Page - Scientific background :

Histamine, acts via distinct histamine H1, H2, H3, and H4 receptors, and regulates various physiological and pathological processes. Hence the activity of Histamine receptors is very crucial. They are members of G-protein coupled receptors superfamily. Thus, have seven transmembrane domains, extracellular N-terminal tail and a cytoplasmic C-terminus1.H1 receptor is excitatory and couples to Gq-type proteins, activating a signaling cascade which eventually leads to an increased cellular concentration of Ca2+ 3.H1 receptor is implicated in type I hypersensitivity allergic reactions caused by numerous allergens1. An allergic reaction occurs when allergens activate mast cells that release histamine, the released histamine binds to H1R and this causes an activation of a cascade that leads to vasodilation and vascular hyper-permeability, resulting in allergic inflammation.H1R is expressed in various tissues throughout body, including the airway, vascular smooth muscle, and brain2. H1R expressed in the brain plays an important role in the regulation of sleep-arousal cycle and memory by binding histamine where it acts as a neurotransmitter1.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Histamine Receptor H1, H1R

Short Description :

A Rabbit Polyclonal Antibody to Histamine H1 Receptor

Long Description :

Anti-Histamine H1 Receptor/HRH1 (extracellular) Antibody (#AHR-006) is a highly specific antibody directed against an epitope of mouse H1R. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize H1R from rat, mouse, and human samples.

Negative Control :

BLP-HR006

Positive Control :

Synonyms :

Histamine Receptor H1, H1R

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HRH1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage