Anti-Histamine H1 Receptor/HRH1 (extracellular)-FITC Antibody | Alomone Labs AHR-006-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Histamine H1 Receptor/HRH1 (extracellular)-FITC Antibody

catalog :

AHR-006-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of Histamine H1 Receptor by direct flow cytometry in live intact mouse P815 mastocytoma cells:

image 2 :

Cell surface detection of Histamine H1 Receptor by direct flow cytometry in live intact human MEG-01 megakaryoblastic leukemia cells:

product information

CAT :

AHR-006-F

SKU :

AHR-006-F_15 mcl

Product Name :

Anti-Histamine H1 Receptor/HRH1 (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P70174

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-HR006

Homology :

Mouse and rat - 15/16 amino acid residues identical; human - 14/16 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

2nd extracellular loop.

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)REDK(S)ETDFYNVTWFK, corresponding to amino acid residues 176 - 191 of mouse H1 Histamine Receptor

Product Page - Scientific background :

Histamine, acts via distinct histamine H1, H2, H3, and H4 receptors, and regulates various physiological and pathological processes. Hence the activity of Histamine receptors is very crucial. They are members of G-protein coupled receptors superfamily. Thus, have seven transmembrane domains, extracellular N-terminal tail and a cytoplasmic C-terminus1.H1 receptor is excitatory and couples to Gq-type proteins, activating a signaling cascade which eventually leads to an increased cellular concentration of Ca2+ 3.H1 receptor is implicated in type I hypersensitivity allergic reactions caused by numerous allergens1. An allergic reaction occurs when allergens activate mast cells that release histamine, the released histamine binds to H1R and this causes an activation of a cascade that leads to vasodilation and vascular hyper-permeability, resulting in allergic inflammation.H1R is expressed in various tissues throughout body, including the airway, vascular smooth muscle, and brain2. H1R expressed in the brain plays an important role in the regulation of sleep-arousal cycle and memory by binding histamine where it acts as a neurotransmitter1.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Histamine Receptor H1, H1R

Short Description :

A Rabbit Polyclonal Antibody to H1R (extracellular) conjugated to the fluorescent dye FITC

Long Description :

Anti-Histamine H1 Receptor/HRH1 (extracellular) Antibody (#AHR-006) is a highly specific antibody directed against an extracellular epitope of the mouse H1R. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize H1R from mouse, rat and human samples. Anti-Histamine H1 Receptor/HRH1 (extracellular)-FITC Antibody (#AHR-006-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize H1R from mouse, rat and human samples.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Histamine Receptor H1, H1R

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HRH1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AHR-006), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage