Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody | Alomone Labs AHR-004 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody

catalog :

AHR-004

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of human chronic myelogenous leukemia (K562) (lanes 1 and 3) and human promyelocytic leukemia (HL-60) (lanes 2 and 4) cell lysates: - 1,2. Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody (#AHR-004), (1:400).3,4. Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody, preincubated with Human Histamine H4 Receptor/HRH4 (extracellular) Blocking Peptide (#BLP-HR004).

image 2 :

Peptide HTLFEWDFGKEIC corresponding to amino acids 75-87 of human HRH4 (AccessionQ9H3N8). 1stextracellular loop.

image 3 :

Cell surface detection of HistamineH4receptorin live intact Jurkat (acute T-cell leukemia) cell lines: - ___Unstained cells + goat-anti-rabbit-PE.___Cells+Anti-Human Histamine H4Receptor (HRH4) (extracellular) Antibody(#AHR-004) (1:20) + goat-anti-rabbit-PE.

product information

CAT :

AHR-004

SKU :

AHR-004-CF_0.2 ml

Product Name :

Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9H3N8

Applications :

IFC LCI WB

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-HR004

Homology :

Won't recognize rat or mouse H4 Histamine Receptor

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

HTLFEWDFGKEIC, corresponding to amino acids 75-87 of human HRH4

Product Page - Scientific background :

Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1The biological effects of histamine are mediated through four receptors (H1- H4 receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.Histamine H4 receptor couples to Gi/G0 proteins and receptor activation leads to inhibition of adenylate cyclase, mobilisation of calcium from intracellular stores and activation of the mitogen-activated protein kinase (MAPK) cascade.1,2Histamine H4 receptor is largely expressed in haemopoietic cells including mast cells, eosinophils, dendritic cells and T lymphocytes. H4 receptors modulate eosinophil migration and the selective recruitment of mast cells leading to amplification of histamine-mediated immune responses. In addition, H4 receptors are involved in dendritic cell activation and the regulation of T lymphocyte cytokine production.2,3These studies indicate that histamine H4 receptor is an attractive therapeutic target for the treatment of inflammatory disorders, such as allergy, asthma, chronic pruritus and autoimmune diseases.2,3

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

H4R, HH4R, G-protein coupled receptor 105, GPCR105, GPRv53, AXOR35

Short Description :

A Rabbit Polyclonal Antibody to Human Histamine H4 Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the 1st extracellular loop of the human histamine H4 receptor. Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody (#AHR-004) can be used in western blot and indirect flow cytometry applications, and recognizes H4R from human samples.

Negative Control :

BLP-HR004

Positive Control :

Synonyms :

H4R, HH4R, G-protein coupled receptor 105, GPCR105, GPRv53, AXOR35

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HRH4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage