Anti-Histamine H3 Receptor (HRH3) Antibody | Alomone Labs AHR-003 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Histamine H3 Receptor (HRH3) Antibody

catalog :

AHR-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Ritzel R, He J, Li Y, Cao T, Khan N, Shim B, et al. Proton extrusion during oxidative burst in microglia exacerbates pathological acidosis following traumatic brain injury. Glia. 2021;69:746-764 pubmed publisher
Apolloni S, Fabbrizio P, Amadio S, Napoli G, Verdile V, Morello G, et al. Histamine Regulates the Inflammatory Profile of SOD1-G93A Microglia and the Histaminergic System Is Dysregulated in Amyotrophic Lateral Sclerosis. Front Immunol. 2017;8:1689 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-Histamine H3 Receptor (HRH3) Antibody (#AHR-003), (1:200). 3,4. Anti-Histamine H3 Receptor (HRH3) Antibody, preincubated with Histamine H3 Receptor/HRH3 Blocking Peptide (#BLP-HR003).

image 2 :

Expression of Histamine H3 receptor in rat cerebellum - Immunohistochemical staining of rat brain frozen sections with Anti-Histamine H3 Receptor (HRH3) Antibody (#AHR-003), (1:100), (green). A. H3R is particularly expressed in dendrites of Purkinje cells (arrows). B. Staining with mouse anti-parvalbumin (red) detected Purkinje cells and interneurons in the molecular layer. C. Merge of the two images demonstrates that the staining was restricted to dendrites of Purkinje cells. Cell nuclei were labeled with DAPI (blue) as the counterstain.

image 3 :

Expression of Histamine H3receptor in rat cerebellum - Immunohistochemical staining of rat brain frozen sections withAnti-Histamine H3Receptor (HRH3) Antibody(#AHR-003) (1:100) (green). A.H3R is particularly expressed in dendrites of Purkinje cells (arrows).B. Staining with mouse anti-parvalbumin (red) detected Purkinje cells and interneurons in the molecular layer. C. Merge of the two images demonstrates that the staining was restricted to dendrites of Purkinje cells. Cell nuclei werelabeled with DAPI (blue) as the counterstain.

product information

CAT :

AHR-003

SKU :

AHR-003-CF_0.2 ml

Product Name :

Anti-Histamine H3 Receptor (HRH3) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9QYN8

Applications :

IC IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-HR003

Homology :

Mouse - identical; human 14/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RTRLRLDGGREAGPE, corresponding to amino acids 228-242 of rat HRH3

Product Page - Scientific background :

Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1The biological effects of histamine are mediated through four receptors (H1-H4 receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.H3 receptor couples to Gi/G0 proteins and receptor activation leads to inhibition of adenylate cyclase, activation of the mitogen-activated protein kinase (MAPK) cascade and inhibition of the Na+/H+ exchanger.1,2H3 receptors are expressed primarily in the central nervous system (CNS) where they are located in presynaptic membranes of histaminergic neurons, where they negatively regulate the synthesis and release of histamine. In addition, H3 receptors are also located on nonhistaminergic neurons, where they regulate the release of other amines such as dopamine, serotonin, and norepinephrine.2,3Based on these studies, a central role for H3 receptors has been proposed in disorders involving cognition such as attention deficit and hyperactivity disorder (ADHD), Alzheimer disease and Schizophrenia, as well as sleep and energy homeostasis (i.e. obesity) disorders.2,3

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

H3R, HH3R, GPCR97

Short Description :

A Rabbit Polyclonal Antibody to Histamine H3 Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat histamine H3 receptor. Anti-Histamine H3 Receptor (HRH3) Antibody (#AHR-003) can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize H3R from rat, mouse and human samples.

Negative Control :

BLP-HR003

Positive Control :

Synonyms :

H3R, HH3R, GPCR97

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HRH3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage