Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody | Alomone Labs AHR-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody

catalog :

AHR-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Jiang L, Cheng L, Chen H, Dai H, An D, Ma Q, et al. Histamine H2 receptor negatively regulates oligodendrocyte differentiation in neonatal hypoxic-ischemic white matter injury. J Exp Med. 2021;218: pubmed publisher
Chen Y, Sha H, Wang Y, Zhou Q, Bhuiyan P, Li N, et al. Histamine 2/3 receptor agonists alleviate perioperative neurocognitive disorders by inhibiting microglia activation through the PI3K/AKT/FoxO1 pathway in aged rats. J Neuroinflammation. 2020;17:217 pubmed publisher
Xu J, Zhang X, Qian Q, Wang Y, Dong H, Li N, et al. Histamine upregulates the expression of histamine receptors and increases the neuroprotective effect of astrocytes. J Neuroinflammation. 2018;15:41 pubmed publisher
Apolloni S, Fabbrizio P, Amadio S, Napoli G, Verdile V, Morello G, et al. Histamine Regulates the Inflammatory Profile of SOD1-G93A Microglia and the Histaminergic System Is Dysregulated in Amyotrophic Lateral Sclerosis. Front Immunol. 2017;8:1689 pubmed publisher

image

image 1 :

Western blot analysis of rat basophilic leukemia cells (RBL) (lanes 1 and 3) and rat brain lysates (lanes 2 and 4): - 1,2. Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody (#AHR-002), (1:200). 3,4. Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody, preincubated with Histamine H2 Receptor/HRH2 (extracellular) Blocking Peptide (#BLP-HR002).

image 2 :

Expression of Histamine H2 receptor in rat stomach - Immunohistochemical staining of paraffin embedded section of rat stomach using Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody (#AHR-002), (1:100). H2R (brown staining) is expressed in parietal cells (arrows) of the gastric glands. Hematoxilin is used as the counterstain.

image 3 :

Cell surface detection ofHistamine H2receptor in live intact rat basophilic leukemia cell lines: - ___Unstained cells + goat-anti-rabbit-FITC.___Cells+Anti-Histamine H2Receptor (HRH2) (extracellular) Antibody(#AHR-002) (1:20) + goat-anti-rabbit-FITC.

product information

CAT :

AHR-002

SKU :

AHR-002-CF_0.2 ml

Product Name :

Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P25102

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-HR002

Homology :

Mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

RNGTRGGNDTFKC, corresponding to amino acids 161-173 of rat HRH2

Product Page - Scientific background :

Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1,2The biological effects of histamine are mediated through four receptors (H1-H4 receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.H2 receptors couple to adenylate cyclase via Gs, leading to the formation of cAMP and the activation of protein kinase A (PKA).1,2 H2 receptors are expressed in several tissues including the brain, gastrointestinal tract and heart.The most studied physiological role of the H2 receptor is its involvement in the regulation of gastric acid secretion.3 Specific H2 receptor antagonists are currently used for the treatment of acid-related gastrointestinal conditions such as peptic ulcer, gastroesophageal reflux disease and dyspepsia.Involvement of H2 receptors in chronic heart failure and immune regulation has also been suggested.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

H2R, HH2R, Gastric receptor I, Gastric receptor 1

Short Description :

A Rabbit Polyclonal Antibody to Histamine H2 Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat histamine H2 receptor. Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody (#AHR-002) can be used in western blot, indirect flow cytometry, immunohistochemistry, and immunocytochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize H2R from rat and mouse samples. It will not recognize human H2R.

Negative Control :

BLP-HR002

Positive Control :

Synonyms :

H2R, HH2R, Gastric receptor I, Gastric receptor 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HRH2

AB Specifictiy :

Will not recognize human histamine H2 receptor.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage