Anti-Histamine H1 Receptor (HRH1) Antibody | Alomone Labs AHR-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Histamine H1 Receptor (HRH1) Antibody

catalog :

AHR-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Wicher S, Roos B, Teske J, Fang Y, PABELICK C, Prakash Y. Aging increases senescence, calcium signaling, and extracellular matrix deposition in human airway smooth muscle. PLoS ONE. 2021;16:e0254710 pubmed publisher
Emrick J, Mathur A, Wei J, Gracheva E, Gronert K, Rosenblum M, et al. Tissue-specific contributions of Tmem79 to atopic dermatitis and mast cell-mediated histaminergic itch. Proc Natl Acad Sci U S A. 2018;115:E12091-E12100 pubmed publisher
Xu J, Zhang X, Qian Q, Wang Y, Dong H, Li N, et al. Histamine upregulates the expression of histamine receptors and increases the neuroprotective effect of astrocytes. J Neuroinflammation. 2018;15:41 pubmed publisher
Cilz N, Lei S. Histamine facilitates GABAergic transmission in the rat entorhinal cortex: Roles of H1 and H2 receptors, Na+ -permeable cation channels, and inward rectifier K+ channels. Hippocampus. 2017;27:613-631 pubmed publisher

image

image 1 :

Western blot analysis of rat heart membranes (lanes 1 and 3) and rat basophilic leukemia (RBL) cell lysates (lanes 2 and 4): - 1,2. Anti-Histamine H1 Receptor (HRH1) Antibody (#AHR-001), (1:200).3,4. Anti-Histamine H1 Receptor (HRH1) Antibody, preincubated with Histamine H1 Receptor/HRH1 Blocking Peptide (#BLP-HR001).

image 2 :

Expression of Histamine H1 receptor in mouse brain - Immunohistochemical staining of mouse ventromedial hypothalamus (VMH) using Anti-Histamine H1 Receptor (HRH1) Antibody (#AHR-001). A. H1R (green fluorescence) appears in the outline of the VMH nucleus (arrows). B. DAPI (blue) counterstain labels all cell nuclei including VMH.

image 3 :

Peptide (C)HSRQYVSGLHLNRE corresponding to amino acids 396-409 of rat HRH1 (AccessionP31390). 3rdintracellular loop.

product information

CAT :

AHR-001

SKU :

AHR-001-CF_0.2 ml

Product Name :

Anti-Histamine H1 Receptor (HRH1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P31390

Applications :

IC IF IFC IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-HR001

Homology :

Mouse - identical; human, dog - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)HSRQYVSGLHLNRE, corresponding to amino acids 396-409 of rat HRH1

Product Page - Scientific background :

Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1The biological effects of histamine are mediated through four receptors (H1-H4 receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.H1 receptors couple to Gq/G11 proteins leading to phospholipase C activation, inositol phosphate production and calcium mobilization.1H1 receptors are expressed in many cell types including endothelial and smooth muscle cells where they mediate vasodilatation and increased vascular permeability effects. H1R is widely distributed through the brain where it mediates sleep-wake cycles and regulates cognitive functions. The receptor is expressed in several cell populations involved in the immune response, including T-cells and B-cells, monocytes and eosinophiles.2,3H1 anti-histamine compounds have been available for almost thirty years and are among the most commonly used medication for conditions such as allergic inflammation. In addition, H1 anti-histamines are also prescribed for the treatment of insomnia and analgesia.2,3

Applications may also work in :

IC IF IFC IHC WB

Supplier :

Alomone Labs

Target :

H1R, HH1R

Short Description :

A Rabbit Polyclonal Antibody to Histamine H1 Receptor

Long Description :

Anti-Histamine H1 Receptor (HRH1) Antibody (#AHR-001) is a highly specific antibody directed against an epitope of rat H1R. The antibody can be used in western blot, immunocytochemistry, indirect flow cytometry, and immunohistochemstry applications. It has been designed to recognize H1R from rat, mouse, and human samples.

Negative Control :

BLP-HR001

Positive Control :

Synonyms :

H1R, HH1R

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HRH1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage