Anti-OTOP1 Antibody | Alomone Labs AHC-005 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-OTOP1 Antibody

catalog :

AHC-005

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat kidney membranes (lanes 1 and 5), mouse heart lysate (lanes 2 and 6), mouse brain lysate (lanes 3 and 7) and rat tongue lysate (lanes 4 and 8): - 1-4. Anti-OTOP1 Antibody (#AHC-005), (1:200). 5-8. Anti-OTOP1 Antibody, preincubated with OTOP1 Blocking Peptide (#BLP-HC005).

image 2 :

Expression of Otopetrin-1 in mouse hippocampus. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Otopetrin-1 Antibody (#AHC-005), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal CA1 region, showed Otopetrin-1 immunoreactivity (green) in astrocytic profiles (arrows). B. Pre-incubation of the antibody with Otopetrin-1 Blocking Peptide (BLP-HC005), suppressed staining. Cell nuclei are stained with DAPI (blue). P= pyramidal layer, SR = stratum radiatum

product information

CAT :

AHC-005

SKU :

AHC-005-CF_0.2 ml

Product Name :

Anti-OTOP1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q80VM9

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-HC005

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

4th intracellular loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)EKSLDESKNPARKLD, corresponding to amino acid residues 374 - 388 of mouse Otopetrin-1

Product Page - Scientific background :

Otopetrin-1 (Otop-1) is a proton channel involved in sour taste recognition. It belongs to the Otopetrin proton channel family together with Otopetrins-2 and 31. Otop-1 is a dimeric structure, with each subunit forming 12 transmembrane helices divided into structurally similar N- and C-domains2.Taste recognition is very important because it enables to sample chemicals in the environment prior to digestion and hence, plays an important role in feeding and avoiding intoxication2. The sense of sour taste is caused by acids, and the first step in sour taste transduction is an influx of H+ ions through Otop-1 channel into the type three Taste Receptor Cells (TRCs). This leads to a decrease in intracellular pH and operation of a signaling cascade1-3.Otop-1 is specifically expressed in type three taste cells and exhibits the Zn2+ sensitivity that is a key feature observed in the proton current of sour taste cells4.Otop-1 also has a role in regulating intracellular Ca2+ levels required for the formation of otoconia in the vertebrate inner ear. Otoconia are complex calcium carbonate biominerals required for the sensation of gravity5.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Proton Channel OTOP1, Otopetrin-1

Short Description :

A Rabbit Polyclonal Antibody to Otopetrin-1 Channel

Long Description :

Anti-OTOP1 Antibody (#AHC-005) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis and immunohistochemistry. It has been designed to recognize Otopetrin-1 from rat, mouse, and human samples.

Negative Control :

BLP-HC005

Positive Control :

Synonyms :

Proton Channel OTOP1, Otopetrin-1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

OTOP1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage