antibody

Anti-HVCN1 Antibody

AHC-001

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

AHC-001-CF_0.2 ml

Anti-HVCN1 Antibody

Antibodies

Antibodies

Polyclonal

Q3U2S8

IFC WB

Human Rat Mouse

Rabbit

BLP-HC001

Rat - identical; human - 11/13 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Intracellular, N-terminus

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

(C)GDDYHTWNVNYKK, corresponding to amino acid residues 32-44 of mouse HVCN1

Currents measured from voltage-gated proton channels were detected1 long before the channel (HVCN1, also known as HV1 and VSOP) was cloned2,3. HVCN1 has four membrane spanning domains and intracellular N- and C-termini. Interestingly, unlike its voltage-gated ion channel counterparts, HVCN1 has no pore4. Also, functional HVCN1 channels are formed by dimers where each monomer has its own conducting pore, each with its own voltage sensor (voltage sensing occurs similarly to other voltage-gated ion channels)5-8. The fundamental role of HVCN1 is to pump out protons, thereby increasing the intracellular pH. The channel is exclusively selective for H+ and opens upon membrane depolarization, although its open state hugely depends on the pH on both sides of the membrane4. Its role is best described in leukocytes where phosphorylation via PKC on a Thr residue potentiates the activity of the channel and increases its open state, thereby increasing the H+ current across the membrane, in this manner mediating optimal NADPH-oxidase (whose optimal activity is at pH 7.5) required for the production of reactive oxygen species (ROS) necessary for phagocytosis to occur4.Apart from leukocytes, HVCN1 is also expressed in basophils where its activation mediates histamine release4,9. In B cells, it maintains optimal signaling, such that ROS production is maintained high10. HVCN1 was found to regulate human spermatozoa activation. Finally, in the airway mucosa, where it regulates pH, channel gating there, is mostly mediated by differences in pH across the membrane as opposed to the membrane potential4.

IFC WB

Alomone Labs

Hydrogen voltage-gated channel 1, HV1, Voltage sensor domain-only protein, VSOP

A Rabbit Polyclonal Antibody to HVCN1 Channel

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of mouse HVCN1 channel. Anti-HVCN1 Antibody (#AHC-001) can be used in western blot and indirect flow cytometry applications. It has been designed to recognize HVCN1 from mouse, rat and human samples.

BLP-HC001

NA

Hydrogen voltage-gated channel 1, HV1, Voltage sensor domain-only protein, VSOP

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

HVCN1

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

yes

IFC

NA

Western blot analysis

1 µg peptide per 1 µg antibody

Contact Alomone for information

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