Anti-Human GLUT3 (extracellular) Antibody | Alomone Labs AGT-043 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human GLUT3 (extracellular) Antibody

catalog :

AGT-043

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 6), human THP-1 monocytic leukemia cell line lysate (lanes 2 and 7), human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 8), human MEG-01 megakaryoblastic leukemia cell line lysate (lanes 4 and 9) and human Jurkat T-cell leukemia cell line lysate (lanes 5 and 10): - 1-5. Anti-Human GLUT3 (extracellular) Antibody (#AGT-043), (1:500). 6-10. Anti-Human GLUT3 (extracellular) Antibody, preincubated with Human GLUT3 (extracellular) Blocking Peptide (#BLP-GT043).

product information

CAT :

AGT-043

SKU :

AGT-043-CF_0.2 ml

Product Name :

Anti-Human GLUT3 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P11169

Applications :

IFC WB

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-GT043

Homology :

Human onlyThis antibody will also recognize the human paralog GLUT14 /SLC2A14 (Accession Q8TDB8) Homology - 12/13 amino acid residues identical The GLUT14 gene is specific to humans and has an overall 945% amino acid identity to GLUT3

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KEFINKTLTDKGN, corresponding to amino acid residues 39 - 51 of human GLUT3

Product Page - Scientific background :

Glucose transporter 3 (GLUT3), encoded by SLC2A3 gene is a glucose transporter, specifically expressed in neurons.GLUT3 is a uniporter carrier, it binds one molecule of substrate at a time and transports it across the membrane. Transport occurs down the substrate concentration gradient, thereby allowing a constant supply of blood sugar for cell's metabolism. The substrate binding site of GLUT3 is alternatively accessible from either side of the membrane by conformational changes of the protein. Thus, it enables both intake and export of glucose, according to glucose levels in and out of the cell1.In the context of cancer, tumor cells often increase their glucose consumption and lactate production. An increasing number of studies are identifying GLUT3 and GLUT1 as major participants in the accelerated metabolism demonstrated by cancer cells. GLUT3 and GLUT1 are also overexpressed in several types of cancer tumors and hence, efforts toward the elucidation of the structure and function of glucose transporters as targets for diagnosis and new cancer therapeutics are invested2.GLUT3 shares a conserved fold that is made of two domains, N- and C-terminal, and six trans-membrane spanning α-helices each. The N- and C-terminal domains are connected by a third domain, comprising five short intracellular α-helices. The overall protein structure has roughly the shape of the letter "V", with a cavity opened towards the extracellular side of the membrane that allows glucose to reach and bind to the substrate binding site3,4.

Applications may also work in :

IFC WB

Supplier :

Alomone Labs

Target :

Glucose transporter 3, Facilitated glucose transporter member 3, SLC2A3

Short Description :

A Rabbit Polyclonal Antibody to Glucose Transporter 3

Long Description :

Anti-Human GLUT3 (extracellular) Antibody (#AGT-043) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry applications. The antibody has been designed to recognize GLUT3 from human samples only. This antibody will also recognize the human paralog GLUT14 /SLC2A14.

Negative Control :

BLP-GT043

Positive Control :

Synonyms :

Glucose transporter 3, Facilitated glucose transporter member 3, SLC2A3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC2A3

AB Specifictiy :

It will not recognize rat or mouse GLUT3.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage