Anti-Human GLUT3 (extracellular)-FITC Antibody | Alomone Labs AGT-043-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human GLUT3 (extracellular)-FITC Antibody

catalog :

AGT-043-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human

application :

flow cytometry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Herman de Sousa C, Pinheiro A, Paramos de Carvalho D, Costa M, Ferreirinha F, Magalh xe3 es Cardoso T, et al. Opposing Effects of Adenosine and Inosine in Human Subcutaneous Fibroblasts May Be Regulated by Third Party ADA Cell Providers. Cells. 2020;9: pubmed publisher

image

image 1 :

Cell surface detection of GLUT3 in live intact human THP-1 monocytic leukemia cells: Cell surface detection of GLUT3 in live intact human THP-1 monocytic leukemia cells: Black line: Cells. Red line: Cells + Rabbit IgG isotype control-FITC. Green line: Cells + Anti-Human GLUT3 (extracellular)-FITC Antibody (#AGT-043-F), (2.5 µg).

product information

CAT :

AGT-043-F

SKU :

AGT-043-F_15 mcl

Product Name :

Anti-Human GLUT3 (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P11169

Applications :

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-GT043

Homology :

Human only This antibody will also recognize the human paralog GLUT14 /SLC2A14 (Accession Q8TDB8) Homology - 12/13 amino acid residues identical The GLUT14 gene is specific to humans and has an overall 945% amino acid identity to GLUT3

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

1st extracellular loop

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KEFINKTLTDKGN, corresponding to amino acid residues 39 - 51 of human GLUT3

Product Page - Scientific background :

The GLUT protein family is encoded by the SLC2 genes and is a member of the major facilitator superfamily of membrane transporters. GLUTs are recognized as key regulators of whole-body glucose homeostasis, and are responsible for the uptake of carbohydrate-derived glucose by cells. There are 14 GLUT isoforms divided into 3 different group classes based on sequence similarity and structural and functional characteristics1,2.GLUT3 is a low-Km protein responsible for glucose uptake into neurons. It is predominantly expressed in neurons and is commonly called "neuronal glucose transporter". The protein contains 12 membrane-spanning domains, an N-linked glycosylation site in loop 1 responsible for high-affinity transport of glucose, and intracellular NH2- and COOH- termini. In addition, there are several conserved residues and motifs designated "sugar transporter signatures"1,2.GLUT3 levels are high in some malignant glioma cells compared to normal glial cells and therefore may be used as a therapeutic target in some cancer treatment3.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Glucose transporter 3, Facilitated glucose transporter member 3, SLC2A3

Short Description :

A Rabbit Polyclonal Antibody to GLUT3 Conjugated to the Fluorescent Dye FITC

Long Description :

Anti-Human GLUT3 (extracellular) Antibody (#AGT-043) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry applications. The antibody has been designed to recognize GLUT3 from human samples only. This antibody will also recognize the human paralog GLUT14 /SLC2A14. Anti-Human GLUT3 (extracellular)-FITC Antibody (#AGT-043-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Glucose transporter 3, Facilitated glucose transporter member 3, SLC2A3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC2A3

AB Specifictiy :

The antibody will not recognize rat or mouse GLUT3.

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AGT-043), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage