Anti-GLUT1 (extracellular) Antibody | Alomone Labs AGT-041 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GLUT1 (extracellular) Antibody

catalog :

AGT-041

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-GLUT1 (extracellular) Antibody (#AGT-041), (1:400).3,4. Anti-GLUT1 (extracellular) Antibody, preincubated with GLUT1 (extracellular) Blocking Peptide (#BLP-GT041).

product information

CAT :

AGT-041

SKU :

AGT-041-CF_0.2 ml

Product Name :

Anti-GLUT1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P11167

Applications :

IFC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GT041

Homology :

Mouse, human - 14 out of 15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EEFYNQTWNHRYGES, corresponding to amino acid residues 41-55 of rat Glucose transporter 1

Product Page - Scientific background :

Glucose transporter 1 (GLUT1) belongs to the major facilitator superfamily (MFS). It is encoded by SLC2A1, and mediates basal-level cellular uptake of glucose into many tissues. GLUT1 contains 12 membrane-spanning domains with both the amino and carboxyl termini oriented intracellularly. In addition, a single extracellular N-linked glycosylation site is present1.GLUT1 is widely expressed, but it is most abundant in fibroblasts, erythrocytes, and endothelial cells with low levels of expression in muscle, liver, and adipose tissue2.Inactivating mutations of GLUT1, resulting in compromised transport activities for glucose, are associated with diseases as a result of lack of energy supply to the brain3. GLUT1 deficiency syndrome (also known as De Vivo syndrome) is characterized by a spectrum of symptoms including early-onset seizures, microcephaly and retarded development4. In addition, elevated expression levels of GLUT1 have been observed in several cancer types, identifying GLUT1 as an important prognostic indicator for tumorigenesis5.

Applications may also work in :

IFC WB

Supplier :

Alomone Labs

Target :

Glucose transporter 1, Facilitated glucose transporter member 1, SLC2A1

Short Description :

A Rabbit Polyclonal Antibody to Glucose Transporter 1

Long Description :

Anti-GLUT1 (extracellular) Antibody (#AGT-041) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize GLUT1 from human, rat, and mouse samples.

Negative Control :

BLP-GT041

Positive Control :

Synonyms :

Glucose transporter 1, Facilitated glucose transporter member 1, SLC2A1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC2A1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage