Anti-SMCT1 (SLC5A8) Antibody | Alomone Labs AGT-036 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SMCT1 (SLC5A8) Antibody

catalog :

AGT-036

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain lysate (lanes 1 and 4), rat kidney membranes (lanes 2 and 5) and mouse colon membranes (3 and 6): - 1-3. Anti-SMCT1 (SLC5A8) Antibody (#AGT-036), (1:200). 4-6. Anti-SMCT1 (SLC5A8) Antibody, preincubated with SMCT1 (SLC5A8) Blocking Peptide (BLP-GT036).

image 2 :

Expression of SMCT1 in rat supraoptic nucleus. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SMCT1 (SLC5A8) Antibody (#AGT-036), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. SMCT1 immunoreactivity (green) was detected in neuronal profiles (arrows). B. Pre-incubation of the antibody with SMCT1 (SLC5A8) Blocking Peptide (BLP-GT036), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AGT-036

SKU :

AGT-036-CF_0.2 ml

Product Name :

Anti-SMCT1 (SLC5A8) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8N695

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GT036

Homology :

Mouse, rat - 13 out of 15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)GGRKQNLDPRYILTK, corresponding to amino acid residues 544 - 558 of human SMCT1

Product Page - Scientific background :

Sodium-coupled monocarboxylate transporter 1, SLC5A8, is an electrogenic sodium (Na+) and chloride (Cl-)-dependent sodium-coupled solute transporter that belongs to the Na+/glucose co-transporter gene family (SLC5) 1.It is expressed in the colon, ileum, kidney and thyroid gland and it is involved in the transport of a variety of monocarboxylates (short-chain fatty acids including L-lactate, D-lactate, pyruvate, acetate, propionate, valerate and butyrate), lactate, mocarboxylate drugs (nicotinate, benzoate, salicylate and 5-aminosalicylate) and ketone bodies (beta-D-hydroxybutyrate, acetoacetate and alpha-ketoisocaproate) 1.SLC5A8 was found to be silenced in colon cancer, gastric cancer and possibly other cancer types and hence it is a candidate tumor suppressor gene. This is probably mediated through its role in transforming butyrate, a metabolite that is related to these types of cancer 2.The structure of SLC5A8 was predicted based on its homology to sodium-iodide symporter (NIS) and on the fact that it is a part of the Solut Sodium Symporter (SSS) protein family. The structure contains 13 α-helical TM segments, with the N-terminus facing the extracellular medium and the C-terminus facing the cytoplasm, and a conserved sodium binding site 3.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Sodium-Coupled Monocarboxylate Transporter 1, Solute Carrier Family 5 Member 8, Apical Iodide Transporter, AIT

Short Description :

A Rabbit Polyclonal Antibody to SMCT1 (SLC5A8) Transporter

Long Description :

Anti-SMCT1 (SLC5A8) Antibody (#AGT-036) is a highly specific antibody directed against an epitope of the human SLC5A8. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SLC5A8 from mouse, rat and human samples.

Negative Control :

BLP-GT036

Positive Control :

Synonyms :

Sodium-Coupled Monocarboxylate Transporter 1, Solute Carrier Family 5 Member 8, Apical Iodide Transporter, AIT

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC5A8

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage