Anti-SGLT2 (extracellular) Antibody | Alomone Labs AGT-032 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SGLT2 (extracellular) Antibody

catalog :

AGT-032

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Singh D, Singh A, Singh D, Kesavan A, Arora S, Tiwary A, et al. Enhanced oral bioavailability and anti-diabetic activity of canagliflozin through a spray dried lipid based oral delivery: a novel paradigm. Daru. 2020;28:191-208 pubmed publisher
Khemais Benkhiat S, Belcastro E, Idris Khodja N, Park S, Amoura L, Abbas M, et al. Angiotensin II-induced redox-sensitive SGLT1 and 2 expression promotes high glucose-induced endothelial cell senescence. J Cell Mol Med. 2020;24:2109-2122 pubmed publisher
El Daly M, Pulakazhi Venu V, Saifeddine M, Mihara K, Kang S, Fedak P, et al. Hyperglycaemic impairment of PAR2-mediated vasodilation: Prevention by inhibition of aortic endothelial sodium-glucose-co-Transporter-2 and minimizing oxidative stress. Vascul Pharmacol. 2018;109:56-71 pubmed publisher

image

image 1 :

Western blot analysis of mouse kidney lysates (lanes 1 and 3) and rat kidney membranes (lanes 2 and 4): - 1,2. Anti-SGLT2 (extracellular) Antibody (#AGT-032), (1:200).3,4. Anti-SGLT2 (extracellular) Antibody, preincubated with SGLT2 (extracellular) Blocking Peptide (#BLP-GT032).

product information

CAT :

AGT-032

SKU :

AGT-032-CF_0.2 ml

Product Name :

Anti-SGLT2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P53792

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GT032

Homology :

Rat - 15/16 amino acid residues identical; mouse - 14/16 amino acid residues identical; human - 12/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KDPAVGNISST(S)YQPR, corresponding to amino acid residues 242-257 of rat SGLT2

Product Page - Scientific background :

The Na+/glucose symporter 2 (SGLT2) belongs to a family of solute sodium symporters (SSS). SSS proteins cotransport Na+ with sugars, amino acids, inorganic ions and vitamins. Members of this family are important in human physiology and disease.SGLT2 is composed of a central group of seven helices (TM2, TM3, TM4, TM7, TM8, TM9, and TM11) that contribute side-chain interactions for ligand selectivity, along with seven supporting helices that stabilize these central helices. A striking feature is two discontinuous TM helices, TM2 and the symmetrically related TM7, in the center of the protomer1. The NH2 and COOH termini of the transporter are both extracellular.Expression of SGLT2 mRNA occurs exclusively in the kidney and primarily in the first two segments of the proximal tubular system. SGLT2 is capable of reabsorbing about 90% of glucose from the primary urine produced in the kidneys.SGLT2 symports a single Na+ molecule together with a glucose molecule. SGLT2 is selective for Na+ as the energizing cation. Apart from H+ and Li+, no other monovalent cation can replace Na+ to drive glucose transport2.Renal SGLT2 inhibitors are new antidiabetic drugs with an insulin-independent mechanism of action. They pose one remarkable advantage compared with already established antidiabetics by increasing urinary glucose excretion without inducing hypoglycemia and thereby promoting bodyweight reduction3.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Na+/glucose cotransporter 2, Low affinity sodium-glucose cotransporter, SLC5A2

Short Description :

A Rabbit Polyclonal Antibody to SGLT2 Transporter

Long Description :

Anti-SGLT2 (extracellular) Antibody (#AGT-032) is a highly specific antibody directed against an epitope of the rat sodium/glucose cotransporter 2. The antibody can be used in western blot analysis. It has been designed to recognize SGLT2 from human, mouse, and rat samples.

Negative Control :

BLP-GT032

Positive Control :

Synonyms :

Na+/glucose cotransporter 2, Low affinity sodium-glucose cotransporter, SLC5A2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC5A2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage