Anti-SGLT1 (extracellular) Antibody | Alomone Labs AGT-031 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SGLT1 (extracellular) Antibody

catalog :

AGT-031

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Boers R, Boers J, Tan B, van Leeuwen M, Wassenaar E, Sanchez E, et al. Retrospective analysis of enhancer activity and transcriptome history. Nat Biotechnol. 2023;: pubmed publisher
Khemais Benkhiat S, Belcastro E, Idris Khodja N, Park S, Amoura L, Abbas M, et al. Angiotensin II-induced redox-sensitive SGLT1 and 2 expression promotes high glucose-induced endothelial cell senescence. J Cell Mol Med. 2020;24:2109-2122 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-SGLT1 (extracellular) Antibody (#AGT-031), (1:200).3,4. Anti-SGLT1 (extracellular) Antibody, preincubated with SGLT1 (extracellular) Blocking Peptide (#BLP-GT031).

image 2 :

Expression of SGLT1 in rat brain sections - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-SGLT1 (extracellular) Antibody (#AGT-031), (1:400). A. SGLT1 staining in the hippocampal dentate gyrus reveals labeling of cells (red) in the granule layer (vertical arrows) and in hilar neurons (horizontal arrow). B. SGLT1 staining in the rat hypothalamic paraventricular nucleus region (in red), appears in cells lining the wall of the third ventricle (horizontal arrows) and in neurons of the paraventricular nucleus (vertical arrows). Cell nuclei are stained with DAPI (blue).

image 3 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4)brain membranes: - 12.Anti-SGLT1 (extracellular) Antibody (#AGT-031) (1:200).34. Anti-SGLT1 (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

AGT-031

SKU :

AGT-031-CF_0.2 ml

Product Name :

Anti-SGLT1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P5379

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GT031

Homology :

Mouse - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)TDAPIQSYERIRN, corresponding to amino acid residues 13-25 of rat SGLT1

Product Page - Scientific background :

The SGLT-1 (Sodium-glucose linked transporter) is part of the SLC5 family of sodium-glucose transporters. This family has more than 220 members in human and bacterial cells and they are expressed in various tissues such as the gut, kidneys and the central nervous system. In human, these transporters are encoded by eleven genes1. SGLT-1 plays a vital role in carbohydrate metabolism and is mainly located in the brush membrane of mature enterocytes in the small intestine. It absorbs D-galactose and D-glucose derived from ingested food2.SGLT-1 has 14 transmembrane α-helices with both the hydrophobic NH2 and hydrophilic COOH termini facing the extracellular side of the cell. SGLT-1 is a symport type transporter carrying sodium and glucose from extracellular space into the cell's cytoplasm. It is believed that sodium binds to the hydrophobic part of the protein and causes a conformational change that enables sugar to binds to the COOH terminal3. The transporter has a number of consensus sites for glycosylation but none are required for the transporter's function.The transporter's regulation was found to be modulated by PKA and PKC. PKA increases vesicle exocytosis 100-fold. Interestingly, the activation of PKC inhibits rat and rabbit SGLT-1 while stimulates human SGLT-1.Pathologies linked to SGLT-1 include the glucose-galactose mal-absorption (GGM) disease caused by a mutation in the transporter. In mice, SGLT-1 knockout causes an inability to survive on glucose containing diets and a decreased insulin and incretin response to glucose4. SGLT-1 is also related to the successful treatment of secretory diarrhea by oral rehydration.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Na+/glucose cotransporter 1, NAGT, High affinity sodium-glucose cotransporter, SLC5A1

Short Description :

A Rabbit Polyclonal Antibody to SGLT1 Transporter

Long Description :

Anti-SGLT1 (extracellular) Antibody (#AGT-031) is a highly specific antibody directed against an epitope of the rat sodium/glucose cotransporter 1. The antibody can be used in western blot and immunohistochemistry applications. It recognizes an extracellular epitope and is thus ideal for detecting the transporter in living cells. It has been designed to recognize SGLT1 from mouse and rat samples. The antibody is not recommended for use on human samples.

Negative Control :

BLP-GT031

Positive Control :

Synonyms :

Na+/glucose cotransporter 1, NAGT, High affinity sodium-glucose cotransporter, SLC5A1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC5A1

AB Specifictiy :

Not recommended for human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage