Anti-GLUT8 (extracellular) Antibody | Alomone Labs AGT-028 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GLUT8 (extracellular) Antibody

catalog :

AGT-028

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 4), mouse liver membranes (lanes 2 and 5) and mouse testis membranes (lanes 3 and 6): - 1-3. Anti-GLUT8 (extracellular) Antibody (#AGT-028), (1:200). 4-6. Anti-GLUT8 (extracellular) Antibody, preincubated with GLUT8 (extracellular) Blocking Peptide (BLP-GT028).

product information

CAT :

AGT-028

SKU :

AGT-028-CF_0.2 ml

Product Name :

Anti-GLUT8 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JJZ1

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GT028

Homology :

Mouse- 13 out of 14 amino acid residues identical Human -11 out of 14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, 5th loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KLTQSGPSNSSHVG, corresponding to amino acid residues 342 - 355 of rat GLUT8

Product Page - Scientific background :

The Glucose transporter (GLUT) family are transmembrane transporters encoded by the slc2a gene superfamily. They are responsible for the transportation of carbohydrates and other substrates down their concentration gradients across lipid bilayers. GLUT transporters therefore play a pivotal role in cellular energetics and glucose sensing. The GLUT family comprises 14 members, divided into 3 classes (I-III), with specific expression patterns and affinities for hexose sugars1.GLUT8 is a class III glucose transporter predominantly expressed in testis and brain. It consists of 12 transmembrane domains, and a single N-glycosylation site. In heterologous expression systems, glucose transport activity was inhibited by fructose and galactose. The transporter carries an NH2-terminal endosomal/lysosomal targeting motif ([DE]XXXL[LI])2.The in-vivo function of GLUT8 remains to be defined. Studies in slc2a8 knock-out mice showed a subtle phenotype including hypoactive sperm, hyperproliferation of hippocampal cells, and resistance to hepatosteatosis in mice fed with high fructose3.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Glucose Transporter Type 8, Glucose Transporter Type X1, GLUTX1, Solute Carrier Family 2 Member 8, SLC2A8

Short Description :

A Rabbit Polyclonal antibody to GLUT8 (extracellular)

Long Description :

Anti-GLUT8 (extracellular) Antibody (#AGT-028) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot and flow cytometry applications. It has been designed to recognize GLUT8 from mouse, rat and human samples.

Negative Control :

BLP-GT028

Positive Control :

Synonyms :

Glucose Transporter Type 8, Glucose Transporter Type X1, GLUTX1, Solute Carrier Family 2 Member 8, SLC2A8

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC2A8

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage