Anti-GLUT5 (SLC2A5) Antibody | Alomone Labs AGT-025 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GLUT5 (SLC2A5) Antibody

catalog :

AGT-025

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4): - 1,2. Anti-GLUT5 (SLC2A5) Antibody (#AGT-025), (1:400).3,4. Anti-GLUT5 (SLC2A5) Antibody, preincubated with GLUT5/SLC2A5 Blocking Peptide (#BLP-GT025).

image 2 :

Expression of Glucose Transporter 5 in mouse hippocampus and cerebellum - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GLUT5 (SLC2A5) Antibody (#AGT-025), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. GLUT5 staining (green) in hippocampal CA3 region, is detected in cells of the pyramidal layer (P) and in their apical dendrites (arrows). B. GLUT5 staining (green) in cerebellum appears in cells of the Purkinje layer (P) and in their apical dendrites (arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

AGT-025

SKU :

AGT-025-CF_0.2 ml

Product Name :

Anti-GLUT5 (SLC2A5) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P43427

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GT025

Homology :

Mouse - 12/14 amino acid residues identical; human - 11/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KNKVSDVYPEKEEK, corresponding to amino acid residues 477 - 490 of rat Glucose Transporter 5

Product Page - Scientific background :

The GLUT protein family belongs to the solute carrier 2 family (SLC2) and is a member of the major facilitator superfamily of membrane transporters. The GLUT proteins have a fold consisting of two symmetrical six transmembrane helix (TM) bundle. In addition, the proteins contain 12 membrane-spanning domains, an N-linked glycosylation site, and intracellular NH2 and COOH termini, and a several conserved residues and motifs designated "sugar transporter signatures"1,2.Fourteen GLUT isoforms have been identified and are divided into 3 different group classes based on sequence similarity and structural and functional characteristics. GLUT 5,7,9 and 11 belong to class ll group1.The GLUT transporters are uniporters, which facilitate the diffusion of monosaccharides like glucose and fructose across the cell membrane in a concentration-dependent manner. GLUT5 is specific to fructose and is primarily expressed in the small intestine, but lower levels were also detected in the brain, adipose tissue, kidney, testes and skeletal muscle. Several metabolic disorders and cancers are linked to increased GLUT5 expression2.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Glucose transporter 5, Facilitated glucose transporter member 5, Fructose transporter

Short Description :

A Rabbit Polyclonal Antibody to Glucose Transporter 5

Long Description :

Anti-GLUT5 (SLC2A5) Antibody (#AGT-025) is a highly specific antibody directed against an epitope of the rat Glucose Transporter 5 protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GLUT5 from human, rat, and mouse samples.

Negative Control :

BLP-GT025

Positive Control :

Synonyms :

Glucose transporter 5, Facilitated glucose transporter member 5, Fructose transporter

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC2A5

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage