Anti-GLUT4 Antibody | Alomone Labs AGT-024 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GLUT4 Antibody

catalog :

AGT-024

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Wang T, Wang J, Hu X, Huang X, Chen G. Current understanding of glucose transporter 4 expression and functional mechanisms. World J Biol Chem. 2020;11:76-98 pubmed publisher
Ashrafi G, Wu Z, Farrell R, Ryan T. GLUT4 Mobilization Supports Energetic Demands of Active Synapses. Neuron. 2017;93:606-615.e3 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) heart membranes: - 1,2. Anti-GLUT4 Antibody (#AGT-024), (1:400).3,4. Anti-GLUT4 Antibody, preincubated with GLUT4 Blocking Peptide (#BLP-GT024).

image 2 :

Expression of Glucose Transporter 4 in rat olfactory bulb - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-GLUT4 Antibody (#AGT-024), (1:400), followed by goat-anti-rabbit-AlexaFluor-488. GLUT4 staining (green) appears in some glomeruli (“G” and horizontal arrows), but not in others (vertical arrow). Cell nuclei are stained with DAPI (blue).

image 3 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4)heart membranes: - 12.Anti-GLUT4 Antibody (#AGT-024) (1:400).34. Anti-GLUT4 Antibody preincubated with the negative control antigen.

product information

CAT :

AGT-024

SKU :

AGT-024-CF_0.2 ml

Product Name :

Anti-GLUT4 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P14672

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GT024

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KPSTELEYLGPDE, corresponding to amino acid residues 495-507 of human Glucose Transporter 4

Product Page - Scientific background :

The GLUT protein family is a member of the major facilitator superfamily of membrane transporters and is encoded by the SLC2 genes. GLUT proteins contain 12 membrane-spanning domains, an N-linked glycosylation site, and intracellular NH2 and COOH termini. In addition there are several conserved residues and motifs designated "sugar transporter signatures".To date, 14 GLUT isoforms have been identified and divided into 3 different classes based on sequence similarity and structural and functional characteristics. GLUT4 together with GLUT 1-3 and 14 belong to Class I.GLUT4 is an insulin-regulated glucose transporter responsible for insulin-regulated glucose uptake into fat and muscle cells. GLUT4 translocates from intracellular stores to the cell surface, from an inactive site to an active one in order to enhance glucose uptake1.GLUT4 is expressed mainly in adipose tissue and skeletal muscle. It is also detected in brain, kidney and intestine.There is no evidence for mutation in GLUT4 that is linked to metabolic disease in humans. However, GLUT4 polymorphisms are rare and equally common in normoglycaemic individuals and type 2 diabetics. General decrease in GLUT4 levels show signs of diabetes, while a disruption in GLUT4 expression in muscle or adipose tissue might contribute to the development of whole-body insulin resistance in humans2.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Glucose transporter 4, Facilitated glucose transporter member 4, Insulin-responsive glucose transporter type 4, SLC2A4

Short Description :

A Rabbit Polyclonal Antibody to Glucose Transporter 4

Long Description :

Anti-GLUT4 Antibody (#AGT-024) is a highly specific antibody directed against an epitope of the human Glucose Transporter 4 protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GLUT4 from human, rat, and mouse samples.

Negative Control :

BLP-GT024

Positive Control :

Synonyms :

Glucose transporter 4, Facilitated glucose transporter member 4, Insulin-responsive glucose transporter type 4, SLC2A4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC2A4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage