Anti-GLUT4-ATTO Fluor-594 Antibody | Alomone Labs AGT-024-AR product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GLUT4-ATTO Fluor-594 Antibody

catalog :

AGT-024-AR

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 594

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Expression of Glucose Transporter 4 in rat olfactory bulb - Immunohistochemical staining of rat olfactory bulb free floating frozen sections using Anti-GLUT4-ATTO Fluor-594 Antibody (#AGT-024-AR), (1:40). GLUT4 immunoreactivity (red) is strongly detected in some glomeruli (vertical arrows). Lower immunoreactivity in other glomeruli (horizontal arrows) is also observed. Nuclei are stained using DAPI as the counterstain.

product information

CAT :

AGT-024-AR

SKU :

AGT-024-AR_50 mcl

Product Name :

Anti-GLUT4-ATTO Fluor-594 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P14672

Applications :

IF IHC

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GT024

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Intracellular, C-terminus

Label :

ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KPSTELEYLGPDE, corresponding to amino acid residues 495-507 of human Glucose Transporter 4

Product Page - Scientific background :

The GLUT protein family is a member of the major facilitator superfamily of membrane transporters and is encoded by the SLC2 genes. GLUT proteins contain 12 membrane-spanning domains, an N-linked glycosylation site, and intracellular NH2 and COOH termini. In addition, there are several conserved residues and motifs designated "sugar transporter signatures".To date, 14 GLUT isoforms have been identified and divided into 3 different classes based on sequence similarity and structural and functional characteristics. GLUT4 together with GLUT 1-3 and 14 belong to Class I.GLUT4 is an insulin-regulated glucose transporter responsible for insulin-regulated glucose uptake into fat and muscle cells. GLUT4 translocates from intracellular stores to the cell surface, from an inactive site to an active one in order to enhance glucose uptake1.GLUT4 is expressed mainly in adipose tissue and skeletal muscle. It is also detected in brain, kidney and intestine.There is no evidence for mutation in GLUT4 that is linked to metabolic disease in humans. However, GLUT4 polymorphisms are rare and equally common in normoglycaemic individuals and type 2 diabetics. General decrease in GLUT4 levels show signs of diabetes, while a disruption in GLUT4 expression in muscle or adipose tissue might contribute to the development of whole-body insulin resistance in humans2.

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

Glucose transporter 4, Facilitated glucose transporter member 4, Insulin-responsive glucose transporter type 4, SLC2A4

Short Description :

A Rabbit Polyclonal Antibody to Glucose Transporter 4 Conjugated to the Fluorescent Dye ATTO-594

Long Description :

Anti-GLUT4 Antibody (#AGT-024) is a highly specific antibody directed against an epitope of the human Glucose Transporter 4 protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GLUT4 from human, rat, and mouse samples. Anti-GLUT4-ATTO Fluor-594 Antibody (#AGT-024-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-GLUT4-ATTO Fluor-594 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

Negative Control :

BLP-GT024

Positive Control :

Synonyms :

Glucose transporter 4, Facilitated glucose transporter member 4, Insulin-responsive glucose transporter type 4, SLC2A4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC2A4

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody #AGT-024), and Immunohistochemical staining (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:40

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage