Anti-GLUT3 (extracellular) Antibody | Alomone Labs AGT-023 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GLUT3 (extracellular) Antibody

catalog :

AGT-023

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Zhu J, Zhang Z, Jia J, Wang L, Yang Q, Wang Y, et al. Sevoflurane Induces Learning and Memory Impairment in Young Mice Through a Reduction in Neuronal Glucose Transporter 3. Cell Mol Neurobiol. 2020;40:879-895 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1, 2. Anti-GLUT3 (extracellular) Antibody (#AGT-023), (1:400).3, 4. Anti-GLUT3 (extracellular) Antibody, preincubated with GLUT3 (extracellular) Blocking Peptide (#BLP-GT023).

image 2 :

Expression of Glucose transporter 3 in mouse hippocampus and cerebellum - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GLUT3 (extracellular) Antibody (#AGT-023), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. GLUT3 staining (green) in mouse hippocampal dentate gyrus, is detected in interneurons (arrows pointing up) in the Hilus and granule layer (G) (arrow pointing down). B. Staining in mouse cerebellum, is seen in purkinje cells (vertical arrows) and dendrites (horizontal arrows) in the molecular layer (Mol). Cell nuclei were stained with DAPI (blue).

product information

CAT :

AGT-023

SKU :

AGT-023-CF_0.2 ml

Product Name :

Anti-GLUT3 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q07647

Applications :

IF IFC IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GT023

Homology :

Mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KDFLNYTLEERLED, corresponding to amino acid residues 39-52 of rat Glucose Transporter 3

Product Page - Scientific background :

The GLUT protein family is encoded by the SLC2 genes and are a member of the major facilitator superfamily of membrane transporters. GLUTs are recognized as key regulators of whole-body glucose homeostasis, and are responsible for the uptake of carbohydrate-derived glucose by cells. There are 14 GLUT isoforms divided into 3 different group classes based on sequence similarity and structural and functional characteristics1,2.GLUT3 is a low-Km protein responsible for glucose uptake into neurons. It is predominantly expressed in neurons and is commonly called "neuronal glucose transporter". The protein contains 12 membrane-spanning domains, an N-linked glycosylation site in loop 1 responsible for high-affinity transport of glucose, and intracellular NH2- and COOH- termini. In addition, there are several conserved residues and motifs designated "sugar transporter signatures"1,2.GLUT3 levels are high in some malignant glioma cells compared to normal glial cells and therefore may be used as a therapeutic target in some cancer treatment3.

Applications may also work in :

IF IFC IHC WB

Supplier :

Alomone Labs

Target :

Glucose transporter 3, Facilitated glucose transporter member 3, SLC2A3

Short Description :

A Rabbit Polyclonal Antibody to Glucose Transporter 3

Long Description :

Anti-GLUT3 (extracellular) Antibody (#AGT-023) is a highly specific antibody directed against an epitope of the rat Glucose Transporter 3 protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. The antibody has been designed to recognize GLUT3 from rat and mouse samples. It will not recognize GLUT3 from human samples.

Negative Control :

BLP-GT023

Positive Control :

Synonyms :

Glucose transporter 3, Facilitated glucose transporter member 3, SLC2A3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC2A3

AB Specifictiy :

Will not recognize human GLUT3.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage