Anti-GLUT2 (SLC2A2) Antibody | Alomone Labs AGT-022 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GLUT2 (SLC2A2) Antibody

catalog :

AGT-022

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat liver membranes: - 1. Anti-GLUT2 (SLC2A2) Antibody (#AGT-022), (1:400).2. Anti-GLUT2 (SLC2A2) Antibody, preincubated with GLUT2/SLC2A2 Blocking Peptide (#BLP-GT022).

image 2 :

Expression of Glucose Transporter 2 in rat cerebellum and hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-GLUT2 (SLC2A2) Antibody (#AGT-022), (1:120), followed by goat-anti-rabbit-AlexaFluor-488 (green). Cell nuclei are stained with DAPI (blue). A. Staining in cerebellum sections, reveals GLUT2 labeling in Purkinje cells (arrows). B. Staining in hippocampus dentate gyrus, shows labeling of both granule cells (horizontal arrow) and in hilar interneurons (vertical arrows).

product information

CAT :

AGT-022

SKU :

AGT-022-CF_0.2 ml

Product Name :

Anti-GLUT2 (SLC2A2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P12336

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GT022

Homology :

Mouse - identical; human - 8/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EFRKKSGSAPPRK, corresponding to amino acid residues 496-508 of rat Glucose Transporter 2

Product Page - Scientific background :

The GLUT protein family is encoded by SLC2 genes and is a member of the major facilitator superfamily of membrane transporters. The GLUT proteins contain 12 membrane-spanning domains, an N-linked glycosylation site, and intracellular NH2 and COOH termini, in addition to several conserved residues and motifs designated "sugar transporter signatures".Fourteen GLUT isoforms have been identified and divided into 3 different group classes based on sequence similarity and structural and functional characteristics. GLUT2 together with GLUT 1,3,4 and 14 belongs to Class I group1.GLUT2 acts as a facilitative glucose transporter in the liver, pancreas, intestine, kidney, and brain and is able to process high sugar concentrations. Due to the transporter's low affinity and high capacity, it guaranties large bidirectional fluxes of glucose in and out of the cell. GLUT2 also can transport other dietary sugars including fructose and galactose, when they are present in physiological concentrations2.GLUT2 mutations cause a severe and rare syndrome called Fanconi-Bickel, an autosomal recessive disorder in carbohydrate metabolism. Patients with Fanconi-Bickel disorder suffer from hepatomegaly, nephropathy, fasting hypoglycemia, sugar intolerance, and growth retardation. In addition, a GLUT2 polymorphism has been associated with preferences for sugary food2.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Glucose transporter 2, Facilitated glucose transporter member 2

Short Description :

A Rabbit Polyclonal Antibody to Glucose Transporter 2

Long Description :

Anti-GLUT2 (SLC2A2) Antibody (#AGT-022) is a highly specific antibody directed against an epitope of the rat Glucose Transporter 2 protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GLUT2 from human, mouse, and rat samples.

Negative Control :

BLP-GT022

Positive Control :

Synonyms :

Glucose transporter 2, Facilitated glucose transporter member 2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC2A2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage