Anti-GLUT2 (SLC2A2)-ATTO Fluor-488 Antibody | Alomone Labs AGT-022-AG product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GLUT2 (SLC2A2)-ATTO Fluor-488 Antibody

catalog :

AGT-022-AG

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 488

clone name :

reactivity :

mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Expression of Glucose Transporter 2 in rat and mouse brain - Immunohistochemical staining of rat and mouse brain sections using Anti-GLUT2 (SLC2A2)-ATTO Fluor-488 Antibody (#AGT-022-AG). A. GLUT2 staining (green) in rat dentate gyrus is detected in cells of the granule layer (G) and in the hilus (arrows). B. In mouse hippocampal CA3 region, GLUT2 staining (green) appears in cells of the pyramidal layer. DAPI counterstain (blue) is used to stain nuclei.

product information

CAT :

AGT-022-AG

SKU :

AGT-022-AG_50 mcl

Product Name :

Anti-GLUT2 (SLC2A2)-ATTO Fluor-488 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P12336

Applications :

IF IHC

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GT022

Homology :

Mouse - identical; human - 8/13 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Intracellular, C-terminus

Label :

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EFRKKSGSAPPRK, corresponding to amino acid residues 496-508 of rat Glucose Transporter 2

Product Page - Scientific background :

The GLUT protein family is encoded by SLC2 genes and is a member of the major facilitator superfamily of membrane transporters. The GLUT proteins contain 12 membrane-spanning domains, an N-linked glycosylation site, and intracellular NH2 and COOH termini, in addition to several conserved residues and motifs designated "sugar transporter signatures".Fourteen GLUT isoforms have been identified and divided into 3 different group classes based on sequence similarity and structural and functional characteristics. GLUT2 together with GLUT1, 3, 4 and 14 belongs to Class I group1.GLUT2 acts as a facilitative glucose transporter in the liver, pancreas, intestine, kidney, and brain and is able to process high sugar concentrations. Due to the transporter's low affinity and high capacity, it guaranties large bidirectional fluxes of glucose in and out of the cell. GLUT2 also can transport other dietary sugars including fructose and galactose when they are present in physiological concentrations2.GLUT2 mutations cause a severe and rare syndrome called Fanconi-Bickel, an autosomal recessive disorder in carbohydrate metabolism. Patients with Fanconi-Bickel disorder suffer from hepatomegaly, nephropathy, fasting hypoglycemia, sugar intolerance, and growth retardation. In addition, a GLUT2 polymorphism has been associated with preferences for sugary food2.

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

Glucose transporter 2, Facilitated glucose transporter member 2

Short Description :

A Rabbit Polyclonal Antibody to Glucose Transporter 2 Conjugated to the Fluorescent Dye ATTO-488

Long Description :

Anti-GLUT2 (SLC2A2) Antibody (#AGT-022) is a highly specific antibody directed against an epitope of the rat Glucose Transporter 2 protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GLUT2 from human, mouse, and rat samples. Anti-GLUT2 (SLC2A2)-ATTO Fluor-488 Antibody (#AGT-022-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well-known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-GLUT2 (SLC2A2)-ATTO Fluor-488 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AG

Positive Control :

Synonyms :

Glucose transporter 2, Facilitated glucose transporter member 2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC2A2

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody #AGT-022), and immunohistochemical staining (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage