Anti-GLUT1 Antibody | Alomone Labs AGT-021 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GLUT1 Antibody

catalog :

AGT-021

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Barahona M, Llanos P, Recabal A, Escobar Acuña K, Elizondo Vega R, Salgado M, et al. Glial hypothalamic inhibition of GLUT2 expression alters satiety, impacting eating behavior. Glia. 2017;: pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1, 2. Anti-GLUT1 Antibody (#AGT-021), (1:500).3, 4. Anti-GLUT1 Antibody, preincubated with GLUT1 Blocking Peptide (#BLP-GT021).

image 2 :

Expression of Glucose Transporter 1 in mouse striatum and wall of lateral ventricle - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-GLUT1 Antibody (#AGT-021), (1:400), followed by donkey-anti-rabbit-Cy3 secondary antibody (red). GLUT1 staining appears in blood vessels (right-pointing arrows) in the striatum (ST) and in the wall of the lateral ventricle (LV, left-pointing arrow). Cell nuclei were stained with DAPI (blue).

image 3 :

Expression of Glucose Transporter 1 in mouse striatum and wall of lateral ventricle - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections usingAnti-GLUT1 Antibody (#AGT-021) (1:400) followed by donkey-anti-rabbit-Cy3 secondary antibody (red). GLUT1 staining appears in blood vessels (right-pointing arrows) in the striatum (ST) and in the wall of the lateral ventricle (LV left-pointing arrow). Cell nuclei were stained with DAPI (blue).

product information

CAT :

AGT-021

SKU :

AGT-021-CF_0.2 ml

Product Name :

Anti-GLUT1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P11166

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GT021

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)RQGGASQSDKTPEE, corresponding to amino acid residues 468-481 of human Glucose transporter 1

Product Page - Scientific background :

Glucose transporter 1 (GLUT1) belongs to the major facilitator superfamily (MFS), one of the largest and most ubiquitous secondary transporter superfamilies. GLUT1, encoded by SLC2A1, mediates the basal-level cellular uptake of glucose into many tissues. GLUT1 contains 12 membrane-spanning domains with both the amino and carboxyl termini oriented intracellularly. In addition, a single extracellular N-linked glycosylation site is present1.GLUT1 is widely expressed, but it is most abundant in fibroblasts, erythrocytes, and endothelial cells with low levels of expression in muscle, liver, and adipose tissue2.Inactivating mutations of GLUT1, resulting in compromised transport activities for glucose, are associated with diseases as a result of lack of energy supply to the brain3. GLUT1 deficiency syndrome (also known as De Vivo syndrome) is characterized by a spectrum of symptoms including early-onset seizures, microcephaly and retarded development4. In addition, elevated expression levels of GLUT1 have been observed in several cancer types, identifying GLUT1 as an important prognostic indicator for tumorigenesis5.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Glucose transporter 1, Facilitated glucose transporter member 1, SLC2A1

Short Description :

A Rabbit Polyclonal Antibody to Glucose Transporter 1

Long Description :

Anti-GLUT1 Antibody (#AGT-021) is a highly specific antibody directed against an epitope of the human Glucose Transporter 1 protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GLUT1 from human, rat, and mouse samples.

Negative Control :

BLP-GT021

Positive Control :

Synonyms :

Glucose transporter 1, Facilitated glucose transporter member 1, SLC2A1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC2A1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage