Anti-SLC6A15 Antibody | Alomone Labs AGT-016 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SLC6A15 Antibody

catalog :

AGT-016

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4): - 1-2. Anti-SLC6A15 Antibody (#AGT-016), (1:200). 3-4. Anti-SLC6A15 Antibody, preincubated with SLC6A15 Blocking Peptide (BLP-GT016).

image 2 :

Expression of SLC6A15 in mouse hippocampus. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SLC6A15 Antibody (#AGT-016), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal CA3 region, showed SLC6A15 immunoreactivity (green) in neuronal profiles in the pyramidal layer (P, horizontal arrows). B. Pre-incubation of the antibody with SLC6A15 Blocking Peptide (BLP-GT016), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AGT-016

SKU :

AGT-016-CF_0.2 ml

Product Name :

Anti-SLC6A15 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8BG16

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GT016

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)GKNIYRKQSGSPTLD corresponding to amino acid residues 691 - 705 of mouse SLC6A15

Product Page - Scientific background :

Sodium-dependent neutral amino acid transporter B(0)AT2, encoded by SLC6A15 gene, is a member of SLC6 neuro transporters family 1.This transporter mediates sodium-dependent, electrogenic transport of leucine and other branched chain amino acids (BCAA). It was found to be expressed in a small percentage of neurons in the hippocampus, the amygdala and other limbic regions as well as in the olfactory bulb, the cortex and the cerebellum 2. Its structure is composed of twelve transmembrane domains, and the ligand-binding site is conserved between mammals and bacteria 3. Since it is active in regulation of the cellular levels of leucine and other BCAA, its activity is related to food intake, body weight and other obesity-related phenotypes 1.In addition to that, BCAA levels and regulated uptake is important for the proper morphology and functions of different brain areas and indeed, one single nucleotide polymorphism (SNP) in SLC6A15 was found to significantly increase the risk of suffering from depression and this polymorphism was also found to affect hippocampal morphology, integrity, and hippocampus-dependent memory 4.These findings emphasize SLC6A15 as pivotal risk factor for vulnerability to psychiatric diseases and makes it an appealing target for drug design.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Solute carrier family 6 member 15, Sodium-dependent neutral amino acid transporter B(0)AT2, Sodium-and chloride-dependent neurotransmitter transporter NTT73, NTT73, B0AT2, SBAT1

Short Description :

A Rabbit Polyclonal Antibody to SLC6A15 Transporter

Long Description :

Anti-SLC6A15 Antibody (#AGT-016) is a highly specific antibody directed against an intracellular epitope of the mouse SLC6A15. The antibody can be used in western blot and immunohistochemistry. It has been designed to recognize SLC6A15 from mouse, rat and human samples.

Negative Control :

BLP-GT016

Positive Control :

Synonyms :

Solute carrier family 6 member 15, Sodium-dependent neutral amino acid transporter B(0)AT2, Sodium-and chloride-dependent neurotransmitter transporter NTT73, NTT73, B0AT2, SBAT1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC6A15

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage