Anti-SLC6A14 (extracellular) Antibody | Alomone Labs AGT-015 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SLC6A14 (extracellular) Antibody

catalog :

AGT-015

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain (lanes 1 and 3) and colon (lanes 2 and 4) lysates: - 1,2. Anti-SLC6A14 (extracellular) Antibody (#AGT-015), (1:200).3,4. Anti-SLC6A14 (extracellular) Antibody, preincubated with SLC6A14 (extracellular) Blocking Peptide (#BLP-GT015).

image 2 :

Expression of SLC6A14 in mouse RTN - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SLC6A14 (extracellular) Antibody (#AGT-015), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. SLC6A14 immunoreactivity (green) is detected in reticular thalamic nucleus (RTN) cells (arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

AGT-015

SKU :

AGT-015-CF_0.2 ml

Product Name :

Anti-SLC6A14 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9UN76

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GT015

Homology :

Mouse - 12/14 amino acid residues identical; rat - 11/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)PSEQYWNKVALQRS, corresponding to amino acid residues 213-226 of human SLC6A14

Product Page - Scientific background :

SLC614A is an amino acid transporter which is a member of the Solute carrier (SLC) 6 transporter family. Currently, the SLC6 family is comprised of >300 prokaryotic and eukaryotic proteins, of which 21 have been identified in humans. SLC6 members have 12 membrane spanning domains (TM) with intracellular N- and C- termini1.SLC614A transports all amino acids (except glutamate and aspartate) into the cell. It has a uni-directional function and it is energized by three different driving forces: a Na+ gradient, a Cl- gradient and the membrane potential. These specific characteristics makes it important in the development of cancerous tumors2. During carcinogenesis, the developing tumor utilizes more resources than healthy tissue and, therefore, there is an over-production of transporters for glucose and amino acids. SLC614A is uniquely suited to the biological needs of tumor cells: Theoretically, this transporter has the ability to concentrate its substrates inside the cell ~1000-fold compared to the extracellular medium, which complies with the increasing demand of the tumor cell for amino acid supply2. Indeed, SLC614A has been shown to be up regulated in multiple cancer types such as, cervical cancer3, breast cancer4, and pancreatic cancer5, as well as the development of Cystic fibrosis6. Hence, the therapeutic potential in affecting the activity of this transporter is clinically significant.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Sodium and chloride-dependent neutral and basic amino acid transporter B(0+), Amino acid transporter ATB0+, Solute carrier family 6 member 14

Short Description :

A Rabbit Polyclonal Antibody to SLC6A14 Transporter

Long Description :

Anti-SLC6A14 (extracellular) Antibody (#AGT-015) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SLC6A14 from rat, mouse, and human samples.

Negative Control :

BLP-GT015

Positive Control :

Synonyms :

Sodium and chloride-dependent neutral and basic amino acid transporter B(0+), Amino acid transporter ATB0+, Solute carrier family 6 member 14

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC6A14

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage