Anti-SLC6A7 (Proline Transporter) Antibody | Alomone Labs AGT-013 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SLC6A7 (Proline Transporter) Antibody

catalog :

AGT-013

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-SLC6A7 (Proline Transporter) Antibody (#AGT-013), (1:400).3,4. Anti-SLC6A7 (Proline Transporter) Antibody, preincubated with SLC6A7/Proline Transporter Blocking Peptide (#BLP-GT013).

product information

CAT :

AGT-013

SKU :

AGT-013-CF_0.2 ml

Product Name :

Anti-SLC6A7 (Proline Transporter) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P28573

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GT013

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KPLMVHMRKYGGIT, corresponding to amino acid residues 602-615 of rat SLC6A7

Product Page - Scientific background :

Proline transporter (PROT) belongs to a gene family of high affinity Na+- and Cl−-dependent plasma membrane transporters. PROT is a glycosylated protein encoded by the Slc6a7 gene. Its structure contains 12 putative transmembrane domains and is enriched in synaptosomal membrane fraction5.PROT transporter is responsible for the active transporter of L-proline through the plasma membrane by using the transmembrane electrochemical ion gradients. Proline is co-transported with Na+ and Cl− ions with a stoichiometry of 1:2:11-3. Several studies have indicated that in the mammalian CNS, L-proline acts as a putative synaptic regulatory molecule. L-proline shows several properties that are common with characterized amino acid neurotransmitters4. Thus, PROT can be used as an important target for therapeutic and pathological alterations of synaptic function2.PROT transporter is expressed in subpopulations of putative glutamatergic pathways in the mammalian brain. Thus, PROT has a physiological role in excitatory neurotransmission.Distribution of human PROT protein can be found in the corpus striatum suggesting that PROT is axonally transported from its site of biosynthesis to the nerve terminals in the striatum4,5.Several recent studies have suggested that enkephalins competitively inhibit mammalian PROT through a direct interaction with the transporter protein at or near the substrate binding site1.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Sodium-dependent proline transporter, Brain-specific L-proline transporter, PROT

Short Description :

A Rabbit Polyclonal Antibody to Proline Transporter (SLC6A7)

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Proline transporter. Anti-SLC6A7 (Proline Transporter) Antibody (#AGT-013) can be used in western blot analysis. It has been designed to recognize PROT from human, rat and mouse samples.

Negative Control :

BLP-GT013

Positive Control :

Synonyms :

Sodium-dependent proline transporter, Brain-specific L-proline transporter, PROT

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC6A7

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage