Anti-GlyT2 (SLC6A5) (extracellular) Antibody | Alomone Labs AGT-012 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GlyT2 (SLC6A5) (extracellular) Antibody

catalog :

AGT-012

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Koshy Cherian A, Parikh V, Wu Q, Mao Draayer Y, Wang Q, Blakely R, et al. Hemicholinium-3 sensitive choline transport in human T lymphocytes: Evidence for use as a proxy for brain choline transporter (CHT) capacity. Neurochem Int. 2017;108:410-416 pubmed publisher

image

image 1 :

Western blot analysis of mouse brain (lanes 1 and 4), rat brain (lanes 2 and 5) and rat cerebellum (lanes 3 and 6) lysates: - 1-3. Anti-GlyT2 (SLC6A5) (extracellular) Antibody (#AGT-012), (1:200).4-6. Anti-GlyT2 (SLC6A5) (extracellular) Antibody, preincubated with GlyT2/SLC6A5 (extracellular) Blocking Peptide (#BLP-GT012).

image 2 :

Expression of glycine transporter 2 in mouse brain stem - Immunohistochemical staining of immersion-fixed, free floating mouse brain frozen sections using Anti-GlyT2 (SLC6A5) (extracellular) Antibody (#AGT-012), (1:200). A. GlyT2 (red) is expressed in neurons (arrow). B. Dapi staining of cell nuclei (blue) is used as a general cellular marker.

image 3 :

Expression of glycine transporter 2 in mouse brain stem - Immunohistochemical staining of immersion-fixed free floating mouse brain frozen sections using Anti-GlyT2 (SLC6A5) (extracellular) Antibody(#AGT-012) (1:200). A. GlyT2 (red) is expressed in neurons (arrow). B. Dapi staining of cell nuclei (blue) is used as a general cellular marker.

product information

CAT :

AGT-012

SKU :

AGT-012-CF_0.2 ml

Product Name :

Anti-GlyT2 (SLC6A5) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P58295

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GT012

Homology :

Mouse - identical; human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CVIGDHPKIQIKNS, corresponding to amino acid residues 333-346 of rat GlyT2

Product Page - Scientific background :

Apart from its obvious biochemical functions, glycine is also an important inhibitory neurotransmitter. Following depolarization, glycine is released from synaptic vesicles, binds to glycine receptors (GlyRs) on postsynaptic membranes thereby causing hyperpolarization of postsynaptic neurons due to the massive influx of Cl- ions. Glycine is then taken up from the synaptic cleft via the glycine transporters GlyT1 and GlyT21.GlyT1 and GlyT2 belong to the SLC6, Na+/Cl- dependent transporter family, of which members include transporters for GABA, serotonin, dopamine and norepinephrine2. Like all SLC6 members, GlyT1 and GlyT2 have 12 transmembrane domains and intracellular N- and C-terminals. Both can be found in different splice variants3,4. SLC6 transporters undergo post-translational modifications. For instance, GlyT1 and GlyT2 are glycosylated, which is important for their membrane trafficking5. Phosphorylation of these two transporters also takes place in a PKC-dependent manner, which may lead to down regulation of both transporters6. Pharmacologically, GlyT1 and GlyT2 can be differentiated by applying sarcosine which inhibits GlyT1 but not GlyT27.GlyT1 and GlyT2 are broadly expressed in the nervous system; GlyT1 is concentrated in glial cells, while GlyT2 is present in glycenergic neurons in the spinal cord, brainstem and cerebellum1. GlyT1 can also be detected in the pancreas, uterus, stomach, spleen, liver and retina4.GlyT1 has become a target for the treatment of schizophrenia, although a defect of the protein is not directly associated with the disorder. Inhibiting GlyT1 should lead to the increase in glutamatergic pathways, thereby decreasing psychotic effects in schizophrenic individuals. GlyT2 has been associated with hyperekplexia, a motor disorder characterized by neonatal hypertonia and startle reflex8.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Na+/Cl--dependent glycine transporter 2

Short Description :

A Rabbit Polyclonal Antibody to Glycine Transporter 2 (SLC6A5)

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat glycine transporter 2. Anti-GlyT2 (SLC6A5) (extracellular) Antibody (#AGT-012) can be used in western blot and immunohistochemistry analysis and has been designed to recognize GlyT2 from rat, mouse and human samples.

Negative Control :

BLP-GT012

Positive Control :

Synonyms :

Na+/Cl--dependent glycine transporter 2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC6A5

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage