Anti-SLC6A8 Antibody | Alomone Labs AGT-008 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SLC6A8 Antibody

catalog :

AGT-008

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse heart (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat brain (lanes 3 and 6) lysates: - 1-3. Anti-SLC6A8 Antibody (#AGT-008), (1:200).4-6. Anti-SLC6A8 Antibody, preincubated with SLC6A8 Blocking Peptide (#BLP-GT008).

image 2 :

Expression of creatine transporter in rat hippocampus and cortex - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC6A8 Antibody (#AGT-008), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Creatine transporter staining (green) in the hippocampal CA1 region is detected in apical dendrites (arrows). B. Creatine transporter immunoreactivity (green) in cortex is observed in soma and apical dendrites of neurons (arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

AGT-008

SKU :

AGT-008-CF_0.2 ml

Product Name :

Anti-SLC6A8 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P28570

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GT008

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RLAVPPRETWTRQMD, corresponding to amino acid residues 46-60 of rat creatine transporter

Product Page - Scientific background :

Creatine transporter (CRT, SLC6A8) is a member of the superfamily of Na+/Cl--coupled transporters for neurotransmitters and organic osmolytes. Creatine Transporter is responsible transports creatine to the blood-brain barrier and into neurons of the central nervous system (CNS). It plays an important role in the storage and utilization of cellular phosphate-bound energy mediated by creatine kinase. It is essential for maintaining brain creatine levels and central nervous system (CNS) functions1,2.Creatine transporter is expressed in various tissues including brain, retina, skeletal muscle, and heart1. The transporter structure contains 12 membrane-spanning domains, a large extracellular loop containing sites for N-linked glycosylation between the third and fourth transmembrane domains. The amino- and carboxyl-termini of the transporter are intracellular3.Creatine transporter is encoded by the SLC6A8 gene and loss-of-function mutations result in creatine deficiency resulting in cerebral creatine deficiency syndromes (CCDSs). These syndromes are the second-most common cause of chromosome X-linked mental retardation. Creatine concentration in the brain is greatly reduced in these patients and they show various neurodevelopmental disorders, including delays in speech and language development, seizures, mental retardation, autism and epilepsy1.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Creatine transporter, Sodium- and chloride-dependent creatine transporter 1, CT1, CRT, CHOT1

Short Description :

A Rabbit Polyclonal Antibody to Creatine Transporter

Long Description :

Anti-SLC6A8 Antibody (#AGT-008) is a highly specific antibody directed against an epitope of the rat Creatine transporter. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SLC6A8 from rat, mouse, and human samples.

Negative Control :

BLP-GT008

Positive Control :

Synonyms :

Creatine transporter, Sodium- and chloride-dependent creatine transporter 1, CT1, CRT, CHOT1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC6A8

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage