Anti-Vesicular GABA Transporter (VGAT) Antibody | Alomone Labs AGT-005 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Vesicular GABA Transporter (VGAT) Antibody

catalog :

AGT-005

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Koga K, Shimoyama S, Yamada A, Furukawa T, Nikaido Y, Furue H, et al. Chronic inflammatory pain induced GABAergic synaptic plasticity in the adult mouse anterior cingulate cortex. Mol Pain. 2018;14:1744806918783478 pubmed publisher
Zhang J, Yu J, Kannampalli P, Nie L, Meng H, Medda B, et al. MicroRNA-mediated downregulation of potassium-chloride-cotransporter and vesicular ?-aminobutyric acid transporter expression in spinal cord contributes to neonatal cystitis-induced visceral pain in rats. Pain. 2017;158:2461-2474 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human U-87 MG glyoblastoma lysates (lanes 3 and 6): - 1-3. Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGT-005), (1:200).4-6. Anti-Vesicular GABA Transporter (VGAT) Antibody, preincubated with Vesicular GABA Transporter/VGAT Blocking Peptide (#BLP-GT005).

image 2 :

Expression of Vesicular GABA Transporter in rat cerebellum - Immunohistochemical staining of rat frozen cerebellum sections using Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGT-005), (1:100). VGAT staining is shown in red and dapi (blue) was used as a general cellular marker. VGAT appears in the cerebellar pinceau (arrows) and in the soma of Purkinje cells (triangles).

product information

CAT :

AGT-005

SKU :

AGT-005-CF_0.2 ml

Product Name :

Anti-Vesicular GABA Transporter (VGAT) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O35458

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GT005

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Cytoplasmic, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)GEFGGHDKPKITAWE, corresponding to amino acid residues 106-120 of rat vesicular GABA transporter

Product Page - Scientific background :

The superfamily of amino acid transporters includes SLC32, SLC36 and SLC38 families. SLC32 has only one member, the vesicular inhibitory amino acid transporter (VIAAT) or vesicular GABA transporter (VGAT)1.VGAT is responsible for the uptake of GABA and glycine in exchange of protons, and their release from nerve terminals1.The transporter is organized into ten membrane spanning domains, a cytosolic N-terminus and a luminal C-terminal tail2. In many regions in the brain, where it is expressed, it is found constitutively phosphorylated, although the biological significance is currently unknown3.VGAT is expressed on synaptic vesicles of GABAergic and glycinergic neurons4-6. The transporter is also detected in the pituitary gland and in the pancreas7,8.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Vesicular inhibitory amino acid transporter, VIAAT, GABA and glycine transporter, SLC32A1

Short Description :

A Rabbit Polyclonal Antibody to Vesicular GABA Transporter

Long Description :

Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGT-005) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize VGAT from human, mouse, and rat samples.

Negative Control :

BLP-GT005

Positive Control :

Synonyms :

Vesicular inhibitory amino acid transporter, VIAAT, GABA and glycine transporter, SLC32A1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC32A1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage