Guinea pig Anti-Vesicular GABA Transporter (VGAT) Antibody | Alomone Labs AGT-005-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-Vesicular GABA Transporter (VGAT) Antibody

catalog :

AGT-005-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Vergassola M, Olivero G, Cisani F, Usai C, Bossi S, Puliti A, et al. Presynaptic mGlu1 Receptors Control GABAB Receptors in an Antagonist-Like Manner in Mouse Cortical GABAergic and Glutamatergic Nerve Endings. Front Mol Neurosci. 2018;11:324 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), human U-87 MG glyoblastoma lysates (lanes 3 and 7) and human SH-SY5Y brain neuroblastoma lysates (lanes 4 and 8): - 1-4. Guinea pig Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGT-005-GP), (1:200).5-8. Guinea pig Anti-Vesicular GABA Transporter (VGAT) Antibody, preincubated with Vesicular GABA Transporter/VGAT Blocking Peptide (#BLP-GT005).

image 2 :

Expression of VGAT in mouse cerebellum. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Guinea pig Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGT-005-GP), (1:600), followed by goat anti-guinea pig-AlexaFluor-594. A. VGAT immunoreactivity (red) appears in the granule layer (GL) and in “pinceau” structures adjacent to purkinje cells (arrows). B. Pre-incubation of the antibody with Vesicular GABA Transporter/VGAT Blocking Peptide (#BLP-GT005), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AGT-005-GP

SKU :

AGT-005-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-Vesicular GABA Transporter (VGAT) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O35458

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-GT005

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Cytoplasmic, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)GEFGGHDKPKITAWE, corresponding to amino acid residues 106-120 of rat vesicular GABA transporter

Product Page - Scientific background :

The superfamily of amino acid transporters includes SLC32, SLC36 and SLC38 families. SLC32 has only one member, the vesicular inhibitory amino acid transporter (VIAAT) or vesicular GABA transporter (VGAT)1.VGAT is responsible for the uptake of GABA and glycine in exchange of protons, and their release from nerve terminals1.The transporter is organized into ten membrane spanning domains, a cytosolic N-terminus and a luminal C-terminal tail2. In many regions in the brain, where it is expressed, it is found constitutively phosphorylated, although the biological significance is currently unknown3.VGAT is expressed on synaptic vesicles of GABAergic and glycynergic neurons4-6. The transporter is also detected in the pituitary gland and in the pancreas7,8.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Vesicular inhibitory amino acid transporter, VIAAT, GABA and glycine transporter, rGVAT, SLC32A1

Short Description :

A Guinea Pig Polyclonal Antibody to Vesicular GABA Transporter

Long Description :

Guinea pig Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGT-005-GP, formerly AGP-129), raised in guinea pigs, is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize VGAT from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGT-005) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-GT005

Positive Control :

Synonyms :

Vesicular inhibitory amino acid transporter, VIAAT, GABA and glycine transporter, rGVAT, SLC32A1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC32A1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:600

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage