Anti-Vesicular GABA Transporter (VGAT)-ATTO Fluor-594 Antibody | Alomone Labs AGT-005-AR product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Vesicular GABA Transporter (VGAT)-ATTO Fluor-594 Antibody

catalog :

AGT-005-AR

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 594

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Expression of Vesicular GABA Transporter in rat cerebellum - Immunohistochemical staining of rat frozen cerebellum sections using Anti-Vesicular GABA Transporter (VGAT)-ATTO Fluor-594 Antibody (#AGT-005-AR), (1:100). A. VGAT staining is shown in red. B. DAPI is used as a general cellular marker (blue). C. Merge of A and B reveal that VGAT is most intensely expressed in the granule and pyramidal layer (arrow).

image 2 :

Peptide (C)KDFLNYTLEERLED corresponding to amino acid residues 39 - 52 of rat Glucose Transporter 3 (Accession Q07647). Extracellular 1st loop.

product information

CAT :

AGT-005-AR

SKU :

AGT-005-AR_50 mcl

Product Name :

Anti-Vesicular GABA Transporter (VGAT)-ATTO Fluor-594 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O35458

Applications :

IF IHC

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GT005

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Cytoplasmic, N-terminus

Label :

ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)GEFGGHDKPKITAWE, corresponding to amino acid residues 106-120 of rat vesicular GABA transporter

Product Page - Scientific background :

The superfamily of amino acid transporters includes SLC32, SLC36 and SLC38 families. SLC32 has only one member, the vesicular inhibitory amino acid transporter (VIAAT) or vesicular GABA transporter (VGAT)1.VGAT is responsible for the uptake of GABA and glycine in exchange of protons, and their release from nerve terminals1.The transporter is organized into ten membrane spanning domains, a cytosolic N-terminus and a luminal C-terminal tail2. In many regions in the brain, where it is expressed, it is found constitutively phosphorylated, although the biological significance is currently unknown3.VGAT is expressed on synaptic vesicles of GABAergic and glycinergic neurons4-6. The transporter is also detected in the pituitary gland and in the pancreas7,8.

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

Vesicular inhibitory amino acid transporter, VIAAT, GABA and glycine transporter, SLC32A1

Short Description :

A Rabbit Polyclonal Antibody to Vesicular GABA Transporter Conjugated to the Fluorescent Dye ATTO-594

Long Description :

Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGT-005) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize VGAT from human, mouse, and rat samples. Anti-Vesicular GABA Transporter (VGAT)-ATTO Fluor-594 Antibody (#AGT-005-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-Vesicular GABA Transporter (VGAT)-ATTO Fluor-594 Antibody has been tested in immunohistochemical applications and is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

BLP-GT005

Positive Control :

Synonyms :

Vesicular inhibitory amino acid transporter, VIAAT, GABA and glycine transporter, SLC32A1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC32A1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AGT-005), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage