Anti-BGT-1 (SLC6A12) Antibody | Alomone Labs AGT-004 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-BGT-1 (SLC6A12) Antibody

catalog :

AGT-004

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Fuhrer T, Palpagama T, Waldvogel H, Synek B, Turner C, Faull R, et al. Impaired expression of GABA transporters in the human Alzheimer's disease hippocampus, subiculum, entorhinal cortex and superior temporal gyrus. Neuroscience. 2017;351:108-118 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-BGT-1 (SLC6A12) Antibody (#AGT-004), (1:200).3,4. Anti-BGT-1 (SLC6A12) Antibody, preincubated with BGT-1/SLC6A12 Blocking Peptide (#BLP-GT004).

image 2 :

Peptide (C)GEMMDQKDKDQVKD corresponding to amino acid residues 22-35 of rat Betaine/GABA Transporter 1 (AccessionP48056). Intracellular N-terminus.

image 3 :

Western blot analysis of mouse kidney membranes: - 1.Anti-BGT-1 (SLC6A12) Antibody(#AGT-004) (1:200).2. Anti-BGT-1 (SLC6A12) Antibody preincubated with the negative control antigen.

product information

CAT :

AGT-004

SKU :

AGT-004-CF_0.2 ml

Product Name :

Anti-BGT-1 (SLC6A12) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P48056

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GT004

Homology :

Mouse - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)GEMMDQKDKDQVKD, corresponding to amino acid residues 22-35 of rat Betaine/GABA Transporter 1

Product Page - Scientific background :

γ-aminobutyric acid (GABA) transporters (GATs) are essential regulators of the activity in the GABAergic system through their continuous uptake of the neurotransmitter from the synaptic cleft and extrasynaptic space. Four GABA transporters (GAT1, GAT2, GAT3 and BGT1) have been identified. Betaine/GABA transporter (BGT1, SLC6A12) is a member of the Na+- and Cl−-dependent neurotransmitter transporter gene family (SLC6) with a high homology to GAT1 (SLC6A1), GAT2 (SLC6A13) and GAT3 (SLC6A11). They have 12 hydrophobic transmembrane domains (TMDs), a large extracellular loop between TMD3 and 4 with multiple glycosylation sites, and intracellular N- and C-termini1,2.BGT1 expression may vary among species. In the dog, BGT1 mRNA is primarily detected in the kidney while the levels in the brain and liver are considerably lower. The highest BGT1 mRNA levels in the mouse are in the liver, while the highest levels in man are in the kidney followed by the liver3. BGT-1 is also present in the brain, but appears to be at low levels and mostly in the leptomeninges rather than in brain tissue proper4.Homozygous BGT1-deficient mice have normal development and show seizure susceptibility indistinguishable from that in wild-type mice in a variety of seizure threshold models5.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Betaine/GABA transporter 1, Na+/Cl--dependent betaine/GABA transporter

Short Description :

A Rabbit Polyclonal Antibody to Betaine/GABA Transporter 1 (SLC6A12)

Long Description :

Anti-BGT-1 (SLC6A12) Antibody (#AGT-004) is a highly specific antibody directed against an epitope of the rat Betaine/GABA transporter 1. The antibody can be used in western blot analysis. It has been designed to recognize BGT-1 from mouse and rat samples. The antibody is unlikely to recognize the protein from human samples.

Negative Control :

BLP-GT004

Positive Control :

Synonyms :

Betaine/GABA transporter 1, Na+/Cl--dependent betaine/GABA transporter

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC6A12

AB Specifictiy :

Not recommended for human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage