Anti-GABA Transporter 3 (GAT-3) Antibody | Alomone Labs AGT-003 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GABA Transporter 3 (GAT-3) Antibody

catalog :

AGT-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:100; loading ...	Pandya M, Palpagama T, Turner C, Waldvogel H, Faull R, Kwakowsky A. Sex- and age-related changes in GABA signaling components in the human cortex. Biol Sex Differ. 2019;10:5 pubmed publisher
	Fuhrer T, Palpagama T, Waldvogel H, Synek B, Turner C, Faull R, et al. Impaired expression of GABA transporters in the human Alzheimer's disease hippocampus, subiculum, entorhinal cortex and superior temporal gyrus. Neuroscience. 2017;351:108-118 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysate: - 1,2. Anti-GABA Transporter 3 (GAT-3) Antibody (#AGT-003), (1:500).3,4. Anti-GABA Transporter 3 (GAT-3) Antibody, preincubated with GABA Transporter 3/GAT-3 Blocking Peptide (#BLP-GT003).

image 2 :

Peptide (C)REARDKAVHERGH corresponding to amino acid residues 35-47 of rat GAT-3 (AccessionP31647). Intracellular N-terminus.

image 3 :

Peptide (C)DYTWNKDNWTFSP corresponding to amino acid residues 72 - 84 of human GPR65 (Accession Q8IYL9). Extracellular 1st loop.

product information

CAT :

AGT-003

SKU :

AGT-003-CF_0.2 ml

Product Name :

Anti-GABA Transporter 3 (GAT-3) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P31647

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GT003

Homology :

Mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)REARDKAVHERGH, corresponding to amino acid residues 35-47 of rat GAT-3

Product Page - Scientific background :

γ-Aminobutiric acid (GABA) is the major inhibitory neurotransmitter in the mammalian central nervous system. Transmission via GABA can be halted by its reuptake through GABA transporters (GATs). These membrane proteins belong to the super family of slc6 genes which encode transporters responsible for the uptake of dopamine, serotonin, norepinephrine, glycine and GABA in a Na+-dependent manner1,2.To date, four GATs have been identified: GAT-1, GAT-2, GAT-3 and BGT-1. Like all members of the slc6 family, they have twelve transmembrane domains and short intracellular N- and C-termini1. GATs couple the transport of one GABA molecule to 2 Na+ ions and one Cl- ion3.GAT-3 is highly expressed in the brain and is linked to epilepsy4.

Applications may also work in :

Supplier :

Alomone Labs

Target :

SLC6A11, GABT3, Na+/Cl- -dependent GABA transporter 3

Short Description :

A Rabbit Polyclonal Antibody to GABA Transporter 3 (GAT-3)

Long Description :

Anti-GABA Transporter 3 (GAT-3) Antibody (#AGT-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize GAT-3 from rat and mouse samples. The antibody will unlikely recognize the protein from human samples.

Negative Control :

BLP-GT003

Positive Control :

Synonyms :

SLC6A11, GABT3, Na+/Cl- -dependent GABA transporter 3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC6A11

AB Specifictiy :

Not recommended for use with human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage