Anti-GABA Transporter 2 (GAT-2) Antibody | Alomone Labs AGT-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GABA Transporter 2 (GAT-2) Antibody

catalog :

AGT-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-GABA Transporter 2 (GAT-2) Antibody (#AGT-002), (1:200).3,4. Anti-GABA Transporter 2 (GAT-2) Antibody, preincubated with GABA Transporter 2/GAT-2 Blocking Peptide (#BLP-GT002).

image 2 :

Expression of GABA transporter 2 in rat lung - Immunohistochemical staining of rat lung paraffin-embedded sections using Anti-GABA Transporter 2 (GAT-2) Antibody (#AGT-002), (1:100). GAT-2 (brown) is expressed in the respiratory epithelium of the bronchioli. Hematoxilin is used as the counterstain.

image 3 :

Expression of GABA transporter 2 in rat lung - Immunohistochemical staining of rat lung paraffin-embedded sections using Anti-GABA Transporter 2 (GAT-2)Antibody (#AGT-002) (1:100). GAT-2 (brown) is expressed in the respiratory epithelium of the bronchioli. Hematoxilin is used as the counterstain.

product information

CAT :

AGT-002

SKU :

AGT-002-CF_0.2 ml

Product Name :

Anti-GABA Transporter 2 (GAT-2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P31646

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GT002

Homology :

Mouse - 14/16 amino acid residues identical; human - 11/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KVEEDGTLEREQWTNK, corresponding to amino acids residues 23-38 of rat GAT-2

Product Page - Scientific background :

γ-Aminobutiric acid (GABA) is the major inhibitory neurotransmitter in the mammalian central nervous system. Transmission via GABA can be halted by its reuptake through GABA transporters (GATs). These membrane proteins belong to the super family of slc6 genes which encode transporters responsible for the uptake of dopamine, serotonin, norepinephrine, glycine and GABA in a Na+-dependent manner1,2.To date, four GATs have been identified: GAT-1, GAT-2, GAT-3 and BGT-1. Like all members of the slc6 family, they have twelve transmembrane domains and short intracellular N- and C-termini1. GATs couple the transport of one GABA molecule to 2 Na+ ions and one Cl- ion3.GAT-2 is expressed in the brain and in the periphery and is also abundant in the kidney and liver4. Due to its relatively broad expression pattern, suggested roles for GAT-2 include maturation of the nervous system, and regulation of GABA levels among others4.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

SLC6A13, GABT2, Na+/Cl-- dependent GABA transporter 2

Short Description :

A Rabbit Polyclonal Antibody to GABA Transporter 2 (GAT-2)

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat GABA transporter 2. Anti-GABA Transporter 2 (GAT-2) Antibody (#AGT-002) can be used in western blot analysis and immunohistochemistry applications and has been designed to recognize GAT-2 from rat, mouse and human samples.

Negative Control :

BLP-GT002

Positive Control :

Synonyms :

SLC6A13, GABT2, Na+/Cl-- dependent GABA transporter 2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC6A13

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage