Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody | Alomone Labs AGT-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody

catalog :

AGT-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:100; loading ...	Pandya M, Palpagama T, Turner C, Waldvogel H, Faull R, Kwakowsky A. Sex- and age-related changes in GABA signaling components in the human cortex. Biol Sex Differ. 2019;10:5 pubmed publisher
	Poliquin S, Hughes I, Shen W, Mermer F, Wang J, Mack T, et al. Genetic mosaicism, intrafamilial phenotypic heterogeneity, and molecular defects of a novel missense SLC6A1 mutation associated with epilepsy and ADHD. Exp Neurol. 2021;342:113723 pubmed publisher
	Moldavan M, Cravetchi O, Allen C. GABA transporters regulate tonic and synaptic GABAA receptor-mediated currents in the suprachiasmatic nucleus neurons. J Neurophysiol. 2017;118:3092-3106 pubmed publisher
	Fuhrer T, Palpagama T, Waldvogel H, Synek B, Turner C, Faull R, et al. Impaired expression of GABA transporters in the human Alzheimer's disease hippocampus, subiculum, entorhinal cortex and superior temporal gyrus. Neuroscience. 2017;351:108-118 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody (#AGT-001), (1:200).3,4. Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody, preincubated with GABA Transporter 1/GAT-1 (extracellular) Blocking Peptide (#BLP-GT001).

image 2 :

Expression of GABATR in rat cerebellum - Immunohistochemical staining of rat cerebellum using Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody (#AGT-001), (1:200). A. GAT-1 staining is shown in green. B. Neurons expressing gamma amino butyric acid (GABA) were labeled with mouse Anti-parvalbumin (red). C. Merge of the two images demonstrates partial colocalization (arrows). GAT-1 appears both in the soma of Purkinje cells (vertical arrows) and in the pinceau (horizontal arrows).

image 3 :

Expression of GABATR in rat cerebellum - Immunohistochemical staining of rat cerebellum usingAnti-GABA Transporter 1 (GAT-1) (extracellular)Antibody (#AGT-001) (1:200). A. GAT-1 staining is shown in green. B. Neurons expressing gamma amino butyric acid (GABA) were labeled with mouse Anti-parvalbumin (red). C. Merge of the two images demonstrates partial colocalization (arrows). GAT-1 appears both in the soma of Purkinje cells (vertical arrows) and in the pinceau (horizontal arrows).

product information

CAT :

AGT-001

SKU :

AGT-001-CF_0.2 ml

Product Name :

Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P23978

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GT001

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)ERNMHQMTDGLDK, corresponding to amino acids residues 194-206 of rat GAT-1

Product Page - Scientific background :

γ-Aminobutiric acid (GABA) is the major inhibitory neurotransmitter in the mammalian central nervous system. Transmission via GABA can be halted by its reuptake through GABA transporters (GATs). These membrane proteins belong to the super family of slc6 genes which encode transporters responsible for the uptake of dopamine, serotonin, norepinephrine, glycine and GABA in a Na+-dependent manner1,2.To date, four GATs have been identified: GAT-1, GAT-2, GAT-3 and BGT-1. Like all members of the slc6 family, they have twelve transmembrane domains and short intracellular N- and C-termini1. GATs couple the transport of one GABA molecule to 2 Na+ ions and one Cl- ion3. GAT-1 activity can be regulated at the level of its plasma membrane expression which is in part regulated by its trafficking via phosphorylation of residues in intracellular loops1.GAT-1 expression is restricted to the central nervous system, specifically to axon terminals, in the neocortex and in some astrocytic processes4.GAT-1 may be implicated in various psychological and neurological disorders such as schizophrenia, epilepsy and cerebral ischemia1,5-7.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

GABATR, GABT1, GABATHG, SLC6A1, Na+/Cl-- dependent GABA transporter 1

Short Description :

A Rabbit Polyclonal Antibody to GABA Transporter 1 (GAT-1)

Long Description :

Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody (#AGT-001) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GAT-1 from rat, mouse, and human samples.

Negative Control :

BLP-GT001

Positive Control :

Synonyms :

GABATR, GABT1, GABATHG, SLC6A1, Na+/Cl-- dependent GABA transporter 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC6A1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage