Guinea Pig Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody | Alomone Labs AGT-001-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea Pig Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody

catalog :

AGT-001-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):

image 2 :

Expression of GABA Transporter 1 in mouse cerebellum.

image 3 :

Expression of GABA Transporter 1 in rat cerebellum.

product information

CAT :

AGT-001-GP

SKU :

AGT-001-GP-CF_0.2 ml

Product Name :

Guinea Pig Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P23978

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-GT001

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)ERNMHQMTDGLDK, corresponding to amino acids residues 194-206 of rat GAT-1

Product Page - Scientific background :

γ-Aminobutiric acid (GABA) is the major inhibitory neurotransmitter in the mammalian central nervous system. Transmission via GABA can be halted by its reuptake through GABA transporters (GATs). These membrane proteins belong to the super family of slc6 genes which encode transporters responsible for the uptake of dopamine, serotonin, norepinephrine, glycine and GABA in a Na+-dependent manner1,2.To date, four GATs have been identified: GAT-1, GAT-2, GAT-3 and BGT-1. Like all members of the slc6 family, they have twelve transmembrane domains and short intracellular N- and C-termini1. GATs couple the transport of one GABA molecule to 2 Na+ ions and one Cl- ion3. GAT-1 activity can be regulated at the level of its plasma membrane expression which is in part regulated by its trafficking via phosphorylation of residues in intracellular loops1.GAT-1 expression is restricted to the central nervous system, specifically to axon terminals, in the neocortex and in some astrocytic processes4.GAT-1 may be implicated in various psychological and neurological disorders such as schizophrenia, epilepsy and cerebral ischemia1,5-7.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

GABATR, GABT1, GABATHG, Na+/Cl-- dependent GABA transporter 1, Solute Carrier Family 6 Member 1, SLC6A1

Short Description :

A Guinea Pig Polyclonal Antibody to GABA Transporter 1 (GAT-1) (extracellular)

Long Description :

Guinea Pig Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody (#AGT-001-GP) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GAT-1 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody (#AGT-001) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Negative Control :

BLP-GT001

Positive Control :

Synonyms :

GABATR, GABT1, GABATHG, Na+/Cl-- dependent GABA transporter 1, Solute Carrier Family 6 Member 1, SLC6A1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC6A1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage