Anti-GPR173 Antibody | Alomone Labs AGR-093 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR173 Antibody

catalog :

AGR-093

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of new born rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):

image 2 :

Western blot analysis of human SHSY-5Y neuroblastoma cell lysate (lanes 1 and 4), mouse BV-2 microglia cell lysate (lanes 2 and 5) and human HMC3 microglial cell line lysate (lanes 3 and 6):

image 3 :

Expression of GPR173 in mouse hippocampus

product information

CAT :

AGR-093

SKU :

AGR-093-CF_0.2 ml

Product Name :

Anti-GPR173 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q6PI62

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR093

Homology :

Rat, human - identical.

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, 3rd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)GMDEVKGEKQLGR, corresponding to amino acid residues 274 - 286 of mouse GPR173

Product Page - Scientific background :

GPR173, also referred to as SREB3 (Super Conserved Receptor Expressed in Brain 3), is a G-protein-coupled receptor (GPCR) belonging to the superfamily of Class A GPCRs. GPR173 is primarily localized to the cell membrane and is prominently expressed in the brain and gonads, particularly within hypothalamic kisspeptin and gonadotropin-releasing hormone (GnRH) neurons1,2. The receptor is activated by Phoenixin (PNX), a neuropeptide involved in reproductive and hormonal regulation, as well as by GnRH-(1–5). PNX binding to GPR173 triggers the cAMP/PKA and MAPK signaling pathways, leading to increased expression of genes regulating reproduction, such as GnRH and Kiss1. On the other hand, GnRH-(1–5) activates GPR173 via the β-arrestin pathway, demonstrating its capacity for biased agonism2,3. Beyond reproduction, GPR173 plays a significant role in modulating inflammatory and oxidative stress pathways. Inflammatory stimuli such as lipopolysaccharide (LPS) reduce GPR173 expression in astrocytes, microglia, and dental pulp cells. Activation of GPR173 by PNX-14 or PNX-20 has been shown to suppress pro-inflammatory cytokines and chemokines, as well as reduce oxidative stress by decreasing ROS production and increasing antioxidant enzymes such as superoxide dismutase (SOD)3,4,6,7. GPR173 also attenuates LPS-induced endoplasmic reticulum (ER) stress by inhibiting the eIF-2α/ATF4/CHOP signaling pathway, preventing the activation of the NLRP3 inflammasome and the subsequent production of IL-1β and IL-18. These protective effects extend to reducing mitochondrial dysfunction, cell death, and infarct volume in neuroinflammatory and ischemic models3,4,7,8. In vivo studies with siRNA knockdown of Gpr173 have shown significant disruptions in estrous cycling and reproductive hormone signaling, confirming its essential role in maintaining reproductive function. Dysregulation of GPR173 has also been linked to neuroinflammation, oxidative stress, and cell death, suggesting potential therapeutic implications for neurodegenerative diseases, stroke, and inflammatory disorders4,7,8.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

G-Protein Coupled Receptor 173, Super Conserved Receptor Expressed In Brain 3, SREB3

Short Description :

A Rabbit Polyclonal Antibody to GPR173

Long Description :

Anti-GPR173 Antibody (#AGR-093) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPR173 from mouse, rat and human samples.

Negative Control :

BLP-GR093

Positive Control :

Synonyms :

G-Protein Coupled Receptor 173, Super Conserved Receptor Expressed In Brain 3, SREB3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPR173

AB Specifictiy :

This antibody is specific for GPR173, it will not recognize the highly homologous receptor GPR85 (SREB2).

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Standard quality control of each lot :

Western blot analysis.

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

more info or order :

Alomone Labs product webpage