Anti-GPR143 Antibody | Alomone Labs AGR-083 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR143 Antibody

catalog :

AGR-083

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):

image 2 :

Western blot analysis of rat eye lysate:

image 3 :

Western blot analysis of human ARPE-19 retinal cell epithelium cell line lysate (lanes 1 and 3) and Malme-3M melanoma cell line lysate (lanes 2 and 4):

product information

CAT :

AGR-083

SKU :

AGR-083-CF_0.2 ml

Product Name :

Anti-GPR143 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P70259

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR083

Homology :

Rat- identical; human - 12 out of 15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, 3rd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KGRKGVYTENERLMG, corresponding to amino acid residues 225 - 239 of mouse GPR143

Product Page - Scientific background :

G-protein coupled receptor 143, GPR143, also known as ocular albinism type 1 protein, OA1, is an orphan GPCR expressed intracellularly in pigment-producing cells on the melanosomal membrane and serves to regulate melanosome size and mediate protein transport to the melanosome via the endolysosomal system.1GPR143 is encoded by the OA1 gene, composed of 404 amino acids with 7 transmembrane helices, and associates with several Gα and Gβ subunits, as well β-Arrestin. Potential ligands for GPR143 include L-3,4-dihydroxyphenylalanine (L-DOPA) and dopamine. High dose L-DOPA treatment leads to intracellular Ca2+ influx and β-Arrestin recruitment. GPR143 has two sorting signals, a dileucine motif in ICL3 and a tryptophan-glutamic acid doublet in the C-terminal tail, which are necessary for lysosomal and melanosomal localization. Functional components of endosomal sorting complexes required for transport (ESCRT) are essential for intracellular sorting and ubiquitination. Due to the unique localization of GPR143 within the cell and its topological orientation, ligands are likely required to bind from the organelle lumen.1GPR143 is largely expressed on the melanosomal membrane of melanocytes, the pigment producing cells that are responsible for producing melanin in the skin and eyes. Melanin is a pigment that is crucial for the protection of skin and eyes from damaging UV light, as well as for the normal development of the optic system and eye function. Mutations in the OA1 gene and its subsequent protein products lead to ocular albinism due to melanosome malformation. Albinism is a pigment disorder characterized by decreased melanin in affected tissues, which leads to adverse effects including foveal hypoplasia and chiasmal misrouting.1,3GPR143 pathophysiology is also implicated in various degenerative diseases, including macular degeneration and Parkinson's disease, as well as playing a possible role in cancer susceptibility and progression.1,2

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

G-Protein Coupled Receptor 143, Ocular Albinism Type 1 Protein, OA1

Short Description :

A Rabbit Polyclonal antibody to GPR143

Long Description :

Anti-GPR143 Antibody (#AGR-083) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPR143 from rat, mouse and human samples.

Negative Control :

BLP-GR083

Positive Control :

Synonyms :

G-Protein Coupled Receptor 143, Ocular Albinism Type 1 Protein, OA1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPR143

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:400-1:500

more info or order :

Alomone Labs product webpage