Anti-GPR142 (extracellular) Antibody | Alomone Labs AGR-082 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR142 (extracellular) Antibody

catalog :

AGR-082

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse pancreas lysate (lanes 1 and 3) and rat pancreas lysate (lanes 2 and 4): - 1-2. Anti-GPR142 (extracellular) Antibody (#AGR-082), (1:400). 3-4. Anti-GPR142 (extracellular) Antibody, preincubated with GPR142 (extracellular) Blocking Peptide (BLP-GR082).

image 2 :

Expression of GPR142 in rat ventromedial hypothalamus. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR142 (extracellular) Antibody (#AGR-082), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. A. GPR142 immunoreactivity (green) appears in glial processes (vertical arrow) and in cells lining the wall of 3rd ventricle (horizontal arrow). B. Pre-incubation of the antibody with GPR142 (extracellular) Blocking Peptide (BLP-GR082), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V= 3rd ventricle.

product information

CAT :

AGR-082

SKU :

AGR-082-CF_0.2 ml

Product Name :

Anti-GPR142 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q7TQN9

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR082

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, 3rd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)APVHRDWRVHLALD, corresponding to amino acid residues 291 - 304 of mouse GPR142

Product Page - Scientific background :

G-protein-coupled receptors (GPCRs) are the largest family of membrane proteins and mediate a myriad of physiological and pathophysiological signaling processes. Of these receptors, all are seven membrane spanning receptors but not all are G protein binding but it is convenient to refer to the receptors as GPCRs1.The GPR142 is a GPCR selectively activated by aromatic amino acids and share 33% amino acid identity with GPR139. L-tryptophan representing one of the most potent ligands, having an EC50 values of 0.2 - 1 mM2. GPR142 is expressed in both rodent and human pancreatic beta cells. Moreover, it was found to be implicated in the regulation of insulin, thereby having a crucial role in Type II diabetes management3. Moreover, GPR142 is highly expressed in immune cells and its ligand L-tryptophan and metabolite serotonin are known to take part in inflammation process. It was shown that mice lacking GPR142 gene are more resilient to anti-type II collagen (CII) antibody-induced arthritis however the exact mechanism is currently unknown4.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

G-Protein Coupled Receptor 142, Probable G-Protein Coupled Receptor 142, PGR2

Short Description :

A Rabbit Polyclonal antibody to GPR142 (extracellular)

Long Description :

Anti-GPR142 (extracellular) Antibody (#AGR-082) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize GPR142 from rat, mouse and human samples.

Negative Control :

BLP-GR082

Positive Control :

Synonyms :

G-Protein Coupled Receptor 142, Probable G-Protein Coupled Receptor 142, PGR2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPR142

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:1000

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage