Anti-GPR158 Antibody | Alomone Labs AGR-078 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR158 Antibody

catalog :

AGR-078

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain lysate ( lanes 1 and 3 ) and rat brain lysate (lanes 2 and 4):

image 2 :

Western blot analysis of human PC-3 prostate adenocarcinoma cell line lysate (lanes 1 and 4), human U-87 MG glioblastoma cell line lysate (lanes 2 and 5) and human OVCAR-3 ovarian carcinoma cell line lysate (lanes 3 and 6):

image 3 :

Expression of GPR158 in rat cerebellum.

product information

CAT :

AGR-078

SKU :

AGR-078-CF_0.2 ml

Product Name :

Anti-GPR158 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8C419

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR078

Homology :

Human - identical, Rat - 14 out of 15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)EPRKPQKSGIMKQQR, corresponding to amino acid residues 960 - 974 of mouse GPR158

Product Page - Scientific background :

G protein-coupled receptor 158 (GPR158) is a protein that belongs to the G protein-coupled receptor (GPCR) superfamily. GPR158 was identified as an orphan receptor and a member of Class C GPCRs. 1Class C GPCRs are obligatory dimers and are characterized by a large N- terminus extracellular ligand-binding domain linked to the seven-transmembrane (7TM) domain, and a large intracellular C-terminus domain. Members of Class C GPCRs include metabotropic glutamate receptors (mGluRs) and GABA(B) receptors, among others.1,2GPR158 is highly expressed in the central nervous system (CNS), particularly in the hippocampus, cortex, midbrain, and cerebellum. Indeed, GPR158 has been linked to affective disorders, such as memory loss, cognitive diseases and depression. 2Recently, osteocalcin (OCN) has been proposed as a putative ligand for GPR158. OCN is is a protein primarily produced by osteoblasts and appears to mediate its memory and cognitive regulation properties, through GPR158.3,4Lately however, GPR158 was identified as the long sought metabotropic receptor mediating the slow neuromodulatory effects of glycine (Gly) and in analogy to the well-known metabotropic glutamate receptors (mGluRs), it has been named as mGlyR.5,6The recognition that GPR158 function is central for cognition and affective states, may pave the way for the development of novel antidepressant drugs targeting GPR158.Finally, GPR158 expression has been found to be elevated in several types of cancer including glioma, prostate and ovarian cancinomas.2

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Probable G-protein coupled receptor 158, Metabotropic Glycine Receptor, mGlyR

Short Description :

A Rabbit Polyclonal antibody to GPR158 receptor

Long Description :

Anti-GPR158 Antibody (#AGR-078) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPR158 from rat, mouse and human samples.

Negative Control :

BLP-GR078

Positive Control :

Synonyms :

Probable G-protein coupled receptor 158, Metabotropic Glycine Receptor, mGlyR

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPR158

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage