Anti-GPRC5B Antibody | Alomone Labs AGR-072 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPRC5B Antibody

catalog :

AGR-072

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):

image 2 :

Western blot analysis of human K562 myelogenous leukemia cell line lysate (lanes 1 and 3) and human NCI-H526 lung carcinoma cell line lysates (lanes 2 and 4):

image 3 :

Expression of GPRC5B in mouse deep cerebellar nuclei.

product information

CAT :

AGR-072

SKU :

AGR-072-CF_0.2 ml

Product Name :

Anti-GPRC5B Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q923Z0

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR072

Homology :

Rat - identical; Human - 15 out of 16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)ENPPNYFDTSQPRMRE, corresponding to amino acid residues 302 - 317 of mouse GPRC5B

Product Page - Scientific background :

GPRC5B is a member of the large G-protein-coupled receptor (GPCR) superfamily of receptors that share a common structure of seven membrane-spanning domains, an extracellular N-terminal domain, an intracellular C-terminal domain, and many conserved residues[1].The ligand-binding domain of GPCRs is variable and the GPCRs have been divided into three classes based on the ligands that stimulate them, as well by key sequence motifs conserved within phylogenetically related subfamily members.GPRC5B (also known as Retinoic Acid-Induced Gene 2 Protein (RAIG2)) is an orphan GPCR most closely related to the Class C GPCR family, based on homology. Class C GPCRs also includes the metabotropic glutamate, calcium-sensing receptor, GABAB, and pheromone receptors.Molecular biology approaches and knockout mouse studies reveal that GPRC5 family proteins have pivotal roles in cancer progression and control of metabolic homeostasis pathways[2].GPCR5B is known to be involved in the regulation of brain homeostasis, proteins related to adhesion or signaling, and remarkably, GPRC5B-mediated tyrosine-phosphorylation signaling cascades play a critical role in development of obesity and insulin resistance through dynamic sphingolipid metabolism[3].GPRC5B contains multiple phosphorylated residues in its carboxyl terminus. Phosphorylation of GPRC5b by the tyrosine kinase Fyn and the subsequent direct interaction with the Fyn Src homology 2 (SH2) domain were found to be critical for the initiation and progression of inflammatory signaling in adipose tissue[4]. For example, a GPRC5B mutant lacking the direct binding site for Fyn failed to activate a positive feedback loop of nuclear factor κB-inhibitor of κB kinase ε signaling.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

G-Protein Coupled Receptor Family C Group 5 Member B, Retinoic Acid-Induced Gene 2 Protein, RAIG2

Short Description :

A Rabbit Polyclonal antibody to GPRC5B receptor

Long Description :

Anti-GPRC5B Antibody (#AGR-072) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPRC5B from rat, mouse and human samples.

Negative Control :

BLP-GR072

Positive Control :

Synonyms :

G-Protein Coupled Receptor Family C Group 5 Member B, Retinoic Acid-Induced Gene 2 Protein, RAIG2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPRC5B

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200-1:800

more info or order :

Alomone Labs product webpage